Regulatory networks define phenotypic classes of human stem cell lines.

Nature
Franz-Josef MüllerJeanne F Loring

Abstract

Stem cells are defined as self-renewing cell populations that can differentiate into multiple distinct cell types. However, hundreds of different human cell lines from embryonic, fetal and adult sources have been called stem cells, even though they range from pluripotent cells-typified by embryonic stem cells, which are capable of virtually unlimited proliferation and differentiation-to adult stem cell lines, which can generate a far more limited repertoire of differentiated cell types. The rapid increase in reports of new sources of stem cells and their anticipated value to regenerative medicine has highlighted the need for a general, reproducible method for classification of these cells. We report here the creation and analysis of a database of global gene expression profiles (which we call the 'stem cell matrix') that enables the classification of cultured human stem cells in the context of a wide variety of pluripotent, multipotent and differentiated cell types. Using an unsupervised clustering method to categorize a collection of approximately 150 cell samples, we discovered that pluripotent stem cell lines group together, whereas other cell types, including brain-derived neural stem cell lines, are very diverse. Using fur...Continue Reading

References

Nov 21, 2001·Nature Reviews. Neuroscience·H I Kornblum, D H Geschwind
Aug 10, 2002·Bioinformatics·Trey IdekerAndrew F Siegel
Nov 9, 2002·Bioinformatics·Olga G TroyanskayaRuss B Altman
Apr 26, 2003·Cloning and Stem Cells·M K CarpenterM S Rao
Dec 4, 2003·Journal of Neuroscience Research·Philip H SchwartzHenry Klassen
Mar 5, 2004·The New England Journal of Medicine·Chad A CowanDouglas A Melton
Mar 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe BrunetJill P Mesirov
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaime ImitolaSamia J Khoury
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Dec 24, 2005·Nature Reviews. Neuroscience·Franz-Josef MüllerJeanne F Loring
Dec 31, 2005·Nucleic Acids Research·Gopa R MishraAkhilesh Pandey
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Arif Murat KocabasJose Bernardo Cibelli
Nov 10, 2006·Nature·Jianlong WangStuart H Orkin
Mar 14, 2007·Molecular Systems Biology·Rob M EwingDaniel Figeys
Apr 6, 2007·BMC Systems Biology·Igor Ulitsky, Ron Shamir
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Jun 19, 2007·Nature Biotechnology·UNKNOWN International Stem Cell InitiativeWeidong Zhang
Oct 20, 2007·Nature Protocols·Melissa S ClineGary D Bader
Nov 22, 2007·Science·Junying YuJames A Thomson
Dec 22, 2007·Nature·Ian ChambersAustin Smith

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Citations

Jan 13, 2011·Archives of Toxicology·Anna M Wobus, Peter Löser
Oct 12, 2012·Stem Cell Reviews and Reports·Peter LöserAndreas Kurtz
Nov 22, 2013·Journal of Proteome Research·Putty-Reddy SudhirChung-Hsuan Chen
Jan 15, 2014·BoneKEy Reports·Ilaria Malanchi
Jul 10, 2009·Nature Biotechnology·Melissa K CarpenterMahendra S Rao
Oct 30, 2012·Nature Cell Biology·Ben D MacArthurIhor R Lemischka
Sep 13, 2012·Nature Communications·Alejandra BrunaCarlos Caldas
Sep 18, 2013·Nature Communications·Michael N C FletcherKerstin B Meyer
Mar 8, 2011·Nature Methods·Franz-Josef MüllerJeanne F Loring
Oct 1, 2011·Nature Methods·Martin F Pera
Apr 10, 2012·Nature Methods·Julia LadewigOliver Brüstle
Feb 6, 2010·Nature Protocols·Igor UlitskyRon Shamir
Sep 10, 2009·Nature Reviews. Molecular Cell Biology·Ben D MacarthurIhor R Lemischka
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph J AbrajanoMark F Mehler
Jun 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Seth A AmentSaurabh Sinha
Jul 24, 2012·Molecular & Cellular Proteomics : MCP·Jing SongRob M Ewing
Oct 13, 2010·Cellular Reprogramming·Anthony W S ChanJin-Jing Yang
Jun 28, 2011·Omics : a Journal of Integrative Biology·Hai FangKankan Wang
Dec 23, 2008·Stem Cells and Development·G Ian Gallicano
Feb 19, 2011·Bioinformatics·Jessica C MarJohn Quackenbush
Jun 26, 2013·Database : the Journal of Biological Databases and Curation·Huilei XuAvi Ma'ayan
Jul 28, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Lena ScheubertGeorg Fuellen
Jan 20, 2009·Nucleic Acids Research·Yonit HalperinRon Shamir
Nov 20, 2009·Nucleic Acids Research·Nuno L Barbosa-MoraisSimon Tavaré
Jun 26, 2010·Nucleic Acids Research·Igor UlitskyRon Shamir
May 3, 2013·Journal of the Royal Society, Interface·Luke MacQueenCraig A Simmons
Feb 6, 2010·Genome Research·Louise LaurentChia-Lin Wei
Mar 31, 2009·Cold Spring Harbor Symposia on Quantitative Biology·B D MacArthurI R Lemischka
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Sep 16, 2011·Science Signaling·Avi Ma'ayan
Jul 9, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael Papetti, Leonard H Augenlicht
Oct 6, 2010·The Journal of Clinical Investigation·Kirsten M L HertoghsRené A W van Lier
Apr 8, 2010·BMC Bioinformatics·Johann M Kraus, Hans A Kestler
Jan 29, 2011·BMC Systems Biology·Thomas R BurkardJacques Colinge

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