Regulatory protein SrpA controls phage infection and core cellular processes in Pseudomonas aeruginosa

Nature Communications
Jiajia YouHongjiang Yang

Abstract

Our understanding of the molecular mechanisms behind bacteria-phage interactions remains limited. Here we report that a small protein, SrpA, controls core cellular processes in response to phage infection and environmental signals in Pseudomonas aeruginosa. We show that SrpA is essential for efficient genome replication of phage K5, and controls transcription by binding to a palindromic sequence upstream of the phage RNA polymerase gene. We identify potential SrpA-binding sites in 66 promoter regions across the P. aeruginosa genome, and experimentally validate direct binding of SrpA to some of these sites. Using transcriptomics and further experiments, we show that SrpA, directly or indirectly, regulates many cellular processes including cell motility, chemotaxis, biofilm formation, pyocyanin synthesis and protein secretion, as well as virulence in a Caenorhabditis elegans model of infection. Further research on SrpA and similar proteins, which are widely present in many other bacteria, is warranted.

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Citations

Nov 30, 2018·The Journal of Clinical Investigation·Yufeng DingJun Qin
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Yang SunZhiming Rao
Nov 8, 2020·Applied and Environmental Microbiology·Xuewei PanZhiming Rao

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Datasets Mentioned

BETA
MEGA7

Methods Mentioned

BETA
PCR
two-hybrid
motility assay
Fluorescent microscopy
flow cytometry
electrophoresis

Software Mentioned

BLASTP
Omicshare
ImageJ
MEGA7
PSI
BLAST
DAVID Bioinformatics Resources
WebLogo
ImageQuant
BLAST ( Iterated BLAST )

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