Regulatory RNA-assisted genome engineering in microorganisms

Current Opinion in Biotechnology
Tong SiHuimin Zhao

Abstract

Regulatory RNAs are increasingly recognized and utilized as key modulators of gene expression in diverse organisms. Thanks to their modular and programmable nature, trans-acting regulatory RNAs are especially attractive in genome-scale applications. Here we discuss the recent examples in microbial genome engineering implementing various trans-acting RNA platforms, including sRNA, RNAi, asRNA and CRISRP-Cas. In particular, we focus on how the scalable and multiplex nature of trans-acting RNAs has been used to tackle the challenges in creating genome-wide and combinatorial diversity for functional genomics and metabolic engineering applications. Advances in computational design and context-dependent regulation are also discussed for their contribution in improving fine-tuning capabilities of trans-acting RNAs.

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Citations

Feb 2, 2016·ACS Synthetic Biology·Sara CletoTimothy K Lu
Sep 24, 2015·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Zehua BaoHuimin Zhao
Dec 29, 2015·Metabolic Engineering·Tadas JakočiūnasJay D Keasling
Feb 21, 2018·Biotechnology and Bioengineering·Mohammad HamediRadHuimin Zhao
Sep 11, 2018·Biotechnology and Bioengineering·Sen YangZhen Kang
Mar 20, 2019·Journal of Industrial Microbiology & Biotechnology·Waqas AhmedRais Ahmed
Jul 28, 2020·Frontiers in Bioengineering and Biotechnology·Yongcan ChenTong Si
Aug 5, 2018·Journal of Industrial Microbiology & Biotechnology·Xin XuQi Li
Nov 18, 2020·Applied Microbiology and Biotechnology·Wen-Hai XieLi-Juan Wang
Jul 30, 2020·Current Opinion in Biotechnology·Mingfeng CaoHuimin Zhao
Apr 9, 2018·Metabolic Engineering·Xia WangShihui Yang

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