Regulatory role of extracellular matrix components in expression of matrix metalloproteinases in cultured hepatic stellate cells

Cell Structure and Function
Y L LiH Senoo

Abstract

Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have assessed the effects of several types of substrata on the expression of MMPs in HSC culture. MMP-1 expression was detectable in HSC culture on polystyrene surface and on type I collagen gel by immunofluorescence staining and reverse transcriptase-polymerase chain reaction (RT-PCR). The results from in situ zymography revealed the presence of interstitial collagenase activity around HSCs and along their cellular processes. Although proMMP-2 and proMMP-9 were detectable by gelatin zymography in the conditioned medium from both cultures using type I collagen gel and Matrigel as substratum, an active form of MMP-2 but not of MMP-9 was detected only in the culture using type I collagen as a substratum. Tissue inhibitor of metalloproteinase-2 expression was observed by RT-PCR in HSCs cultured on or in type I collagen gel, suggesting the suppression of MMP-2 activity detected in HSC culture using type I collagen. These result...Continue Reading

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Citations

Jul 6, 2006·Journal of Molecular Histology·Surya A ReisRob Willemsen
Feb 24, 2012·Digestive Diseases and Sciences·Hiroyuki EdaBradley T Keller
Feb 10, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Van de BovenkampP Olinga
Nov 12, 2010·Cell Biology International·Haruki SenooYoshihiro Mezaki
Nov 25, 2004·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Takashi GotoSumio Watanabe
Oct 29, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Tannin J FujaIngo R Titze
Mar 4, 2015·Cell Adhesion & Migration·Jiří Kanta
Sep 6, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shigeki TsukadaRichard A Rippe
Apr 16, 2005·World Journal of Gastroenterology : WJG·Qing LiYong-Hong Xiao
Jul 27, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Liang WangFei Chen
Jan 29, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Nobuyo Higashi, Haruki Senoo
May 25, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Nobuyo HigashiHaruki Senoo
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Mar 17, 2017·Anatomical Science International·Haruki SenooMutsunori Fujiwara
Nov 24, 2012·Physiological Research·A JiroutováJ Kanta

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