Regulatory roles for the homeodomain and C2H2 zinc finger regions of Cryptococcus neoformans Ste12alphap

Molecular Microbiology
Yun C ChangK J Kwon-Chung

Abstract

The STE12alpha gene of Cryptococcus neoformans encodes a protein containing both homeodomain and zinc finger regions. As homeodomains and zinc finger regions are important domains for the function of many transcription factors, we used site-specific mutagenesis to delineate the roles of these two domains. The homeodomain and zinc finger regions are each important for the function of Ste12alphap. DNA binding ability, mating frequency, and haploid fruiting capability were reduced in strains with mutations in the homeodomain, whereas virulence and capsule size in the mouse brain were increased. In contrast, mutations in the zinc fingers region resulted in decreased virulence, reduced capsule size in the mouse brain and decreased gene expression of capsule associated genes. In addition, phospholipase activity was increased in the zinc finger mutants. Taken together, most of the phenotypes previously observed in the ste12alpha deletion strains were reproduced in these two types of mutants. However, unlike mutations in the homeodomain/zinc finger region, complete deletion of STE12alpha caused a severe reduction in virulence and a decrease in phospholipase activity. These data suggest that region(s) other than the homeodomain and zinc...Continue Reading

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Citations

Oct 19, 2012·Future Microbiology·Wilber Sabiiti, Robin C May
Jun 16, 2009·Molecular Plant-microbe Interactions : MPMI·Nicolas Rispail, Antonio Di Pietro
Jul 16, 2017·Molecular Plant Pathology·Xiaoguo ZhuZhensheng Kang
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May 15, 2019·Annual Review of Microbiology·Youbao ZhaoXiaorong Lin
May 17, 2014·Molecular Plant Pathology·Qin GuZhonghua Ma
Feb 23, 2020·International Journal of Molecular Sciences·Yuan-Hong Li, Tong-Bao Liu

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