Regulatory segments of Ca2+/calmodulin-dependent protein kinases.

The Journal of Biological Chemistry
G ZhiJ T Stull

Abstract

Catalytic cores of skeletal and smooth muscle myosin light chain kinases and Ca2+/calmodulin-dependent protein kinase II are regulated intrasterically by different regulatory segments containing autoinhibitory and calmodulin-binding sequences. The functional properties of these regulatory segments were examined in chimeric kinases containing either the catalytic core of skeletal muscle myosin light chain kinase or Ca2+/calmodulin-dependent protein kinase II with different regulatory segments. Recognition of protein substrates by the catalytic core of skeletal muscle myosin light chain kinase was altered with the regulatory segment of protein kinase II but not with smooth muscle myosin light chain kinase. Similarly, the catalytic properties of the protein kinase II were altered with regulatory segments from either myosin light chain kinase. All chimeric kinases were dependent on Ca2+/calmodulin for activity. The apparent Ca2+/calmodulin activation constant was similarly low with all chimeras containing the skeletal muscle catalytic core. The activation constant was greater with chimeric kinases containing the catalytic core of Ca2+/calmodulin-dependent protein kinase II with its endogenous or myosin light chain kinase regulatory...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·P I Hanson, H Schulman
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F H CruzaleguiA R Means
Aug 13, 1991·Biochimica Et Biophysica Acta·B E Kemp, R B Pearson
Jan 1, 1985·Annual Review of Biochemistry·A C NairnP Greengard
Mar 1, 1989·The Biochemical Journal·R J ColbranT R Soderling
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P T KellyM N Waxham
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L BillingsleyR L Kincaid
May 2, 1985·Nature·Y S BabuW J Cook
Jul 14, 1995·The Journal of Biological Chemistry·J K KruegerJ T Stull
Apr 28, 1995·The Journal of Biological Chemistry·Z H GaoJ T Stull
Feb 3, 1995·The Journal of Biological Chemistry·A Ishida, H Fujisawa
Aug 23, 1993·FEBS Letters·L V DekkerP J Parker
Feb 1, 1997·Journal of Muscle Research and Cell Motility·P J GallagherJ T Stull

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Citations

Jun 22, 2000·Archives of Biochemistry and Biophysics·A P Kwiatkowski, J M McGill
Jul 18, 2002·Trends in Pharmacological Sciences·Linda J Van Eldik
Aug 2, 2003·Fungal Genetics and Biology : FG & B·Maria José A de CarvalhoM Sueli S Felipe
Oct 24, 2006·The Journal of Biological Chemistry·Yijun JinPatricia J Gallagher
May 14, 2008·The Journal of Biological Chemistry·Jian HuangJames T Stull
Jan 15, 1999·Acta Physiologica Scandinavica·J T StullG Zhi
Jun 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Audrey N ChangJames T Stull
Mar 13, 1999·The Journal of Biological Chemistry·C Gu, D M Cooper
Nov 30, 2000·The Journal of Biological Chemistry·K E Kamm, J T Stull
Oct 25, 2002·The Journal of Biological Chemistry·David S ReinerFrances D Gillin
Feb 3, 2011·Archives of Biochemistry and Biophysics·James T StullRene Vandenboom

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