Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species

Plant Molecular Biology
Lynn M KramzarDavid C Higgs

Abstract

The 5' untranslated regions (UTR) of chloroplast mRNAs often contain regulatory sequences that control RNA stability and/or translation. The petD chloroplast mRNA in Chlamydomonas reinhardtii has three such essential regulatory elements in its 362-nt long 5' UTR. To further analyze these elements, we compared 5' UTR sequences from four Chlamydomonas species (C. reinhardtii, C. incerta, C. moewusii and C. eugametos) and five independent strains of C. reinhardtii. Overall, these petD 5' UTRs have relatively low sequence conservation across these species. In contrast, sequences of the three regulatory elements and their relative positions appear partially conserved. Functionality of the 5' UTRs was tested in C. reinhardtii chloroplasts using beta-glucuronidase reporter genes, and the nearly identical C. incerta petD functioned for mRNA stability and translation in C. reinhardtii chloroplasts while the more divergent C. eugametos petD did not. This identified what may be key features in these elements. We conclude that these petD regulatory elements, and possibly the corresponding trans-acting factors, function via mechanisms highly specific and surprisingly sensitive to minor sequence changes. This provides a new and broader persp...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H S ShepherdN W Gillham
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R A RoffeyR T Sayre
May 11, 1989·Nucleic Acids Research·M TurmelA Bergeron
May 1, 1988·Molecular and Cellular Biology·R L Feinbaum, F M Ausubel
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W SakamotoD B Stern
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·R G DragerD B Stern
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·R G DragerD B Stern
Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·F E VaistijJ D Rochaix
Aug 18, 2000·Biochimie·A Barkan, M Goldschmidt-Clermont
Aug 18, 2000·Biochimie·R A MondeD B Stern
Aug 18, 2000·Biochimie·W Zerges
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K L KindleD B Stern
Mar 20, 2004·Nucleic Acids Research·Svetlana A ShabalinaDavid J Lipman

❮ Previous
Next ❯

Citations

Nov 28, 2012·Applied Microbiology and Biotechnology·Javier A Gimpel, Stephen P Mayfield
Jul 31, 2007·Photosynthesis Research·Julia Marín-NavarroStephen P Mayfield
Mar 1, 2009·American Journal of Botany·Joshua J BaeckerMargaret J Hollingsworth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.