PMID: 8594546Oct 1, 1995Paper

Regulatory volume decrease in a renal distal tubular cell line (A6). I. Role of K+ and Cl-

Pflügers Archiv : European journal of physiology
P De SmetW van Driessche

Abstract

Changes in volume of A6 epithelial cells were monitored by recording cell thickness (Tc). The response of Tc to a reduction of the basolateral osmolality from 260 to 140 mosmol/kg was recorded while transepithelial Na+ transport was inhibited by 20 microM amiloride. With Cl--containing bathing media, this osmotic challenge elicited a rapid rise in Tc followed by a regulatory volume decrease (RVD). Substitution of SO4(2-) or gluconate for Cl- markedly reduced the RVD, whereas cells completely maintained their ability to regulate their volume after replacing Cl- by NO3(-). A conductive pathway for Cl- excretion is suggested, which is insensitive to NPPB [5-nitro-2-(3-phenylpropylamino)benzoic acid], an inhibitor of some types of Cl- channels. Ba2+ (5 or 20 mM) reduced the RVD. A more pronounced inhibition of the RVD was obtained with 500 microM quinine, a potent blocker of volume-activated K+ channels. K+-induced depolarization of the basolateral membranes of tissues incubated with SO4(2-)-containing solutions completely abolished the RVD. Noise analysis in the presence of Ba2+ showed the activation of an apical K+ conductive pathway. These results demonstrate that cell volume regulation is controlled by processes involving Cl- a...Continue Reading

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Citations

Dec 22, 1999·Biochemical and Biophysical Research Communications·N NiisatoY Marunaka
Mar 24, 2009·Purinergic Signalling·Scott Sp WildmanBrian F King
Nov 8, 2002·Biochimica Et Biophysica Acta·S SchmiederJ Ehrenfeld
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Jul 11, 2002·American Journal of Physiology. Cell Physiology·Ilse SmetsWilly Van Driessche
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Aug 4, 1998·The American Journal of Physiology·J LiW Van Driessche
Oct 1, 1998·The American Journal of Physiology·J A Hernández, E Cristina
Oct 20, 2007·The Journal of Biological Chemistry·Sébastien L'HostePhillipe Poujeol
Aug 5, 1998·The American Journal of Physiology·P De SmetW Van Driessche
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May 8, 1999·The American Journal of Physiology·T J BirdseyS L Greenwood
Feb 10, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·A Marino, G La Spada

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