PMID: 8594547Oct 1, 1995Paper

Regulatory volume decrease in a renal distal tubular cell line (A6). II. Effect of Na+ transport rate

Pflügers Archiv : European journal of physiology
P De SmetW Van Driessche

Abstract

A6 epithelia, a cell line originating from the distal tubular part of the kidney of Xenopus laevis, were cultured on permeable supports and mounted in an Ussing-type chamber. Cell thickness (Tc), short-circuit current (Isc) and transepithelial conductance (Gt) were recorded while tissues were bilaterally incubated in NaCl solutions and the transepithelial potential was clamped to zero. Effects of inhibition and stimulation of transepithelial Na+ transport on cell volume and on its regulation during a hyposmotic challenge were investigated. Under control conditions a slow spontaneous decrease of Tc described by a linear baseline was recorded. The reduction of the apical osmolality from 260 to 140 mosmol/kg did not alter cell volume significantly, demonstrating a negligible water permeability of the apical barrier. The inhibition of Na+ uptake by replacing apical Na+ by N-methyl-d-glucamine (NMDG+) did not affect cell volume under isotonic conditions. An increase of Tc by 12.1% above the control baseline was recorded after blocking active transport with ouabain for 60 min. The activation of Na+ transport with insulin or oxytocin, which is known to activate the apical water permeability in other epithelia, did not alter cell volum...Continue Reading

References

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Citations

Oct 1, 1995·Pflügers Archiv : European journal of physiology·P De SmetW Van Driessche
Jun 18, 2004·Kidney International·Niaz AhmadJ Peter A Lodge
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Ilse SmetsWilly Van Driessche
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Sep 14, 1999·The American Journal of Physiology·W ZeiskeW van Driessche
Jun 13, 2002·American Journal of Physiology. Renal Physiology·David J RozanskyDavid Pearce
Jun 1, 1997·The American Journal of Physiology·W Van DriesscheI Mountian
Aug 5, 1998·The American Journal of Physiology·P De SmetW Van Driessche

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