Regulatory volume increase of human non-pigmented ciliary epithelial cells

Experimental Eye Research
M M CivanK Peterson-Yantorno

Abstract

Cells (ODM/SV40) derived from human non-pigmented ciliary epithelial cells were studied by electronic cell sizing. After transiently suspending the cells in hypotonic solution, isotonicity was restored by addition of sucrose. The cell volumes (vc) initially fell below those of control isotonic suspensions, and subsequently increased towards the baseline level. This secondary increase in vc is termed the regulatory volume increase (RVI). Results obtained with ionic substitutions and transport inhibitors indicate that four ionic mechanisms can support the RVI in these cells: coupled Na+/H+ and Cl-/HCO3- antiports, a Na+/Cl- symport, a Na+/K+/2Cl- symport, and a Na+ channel in parallel with a Cl-/HCO3- antiport. Arachidonic acid metabolites regulate the RVI very differently from their effects on the regulatory volume response (RVD) of the same cells to cell swelling. Prostaglandin E2 (PGE2), leukotriene (LTD4) and the PKC-inhibitor staurosporine all inhibit the RVI. Blockade of the cyclooxygenase, lipoxygenase and epoxygenase pathways of arachidonic acid metabolism [with 5,8,11,14-eicosatetraynoic acid (ETYA)] produces a net acceleration of the RVI. In contrast, PGE2 and staurosporine stimulate, LTD4 has no effect, and ETYA inhibi...Continue Reading

Citations

Jun 10, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Casto RamosEduardo Soriano
Dec 11, 1997·The Journal of Experimental Zoology·M M CivanM Coca-Prados
Sep 24, 2004·The Journal of Membrane Biology·C W Do, M M Civan
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Oct 6, 2006·American Journal of Physiology. Cell Physiology·Chu Yan ChanChi Ho To
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Yining Hou, Nicholas A Delamere
Aug 31, 2007·American Journal of Physiology. Cell Physiology·Charles W McLaughlinMortimer M Civan
Feb 5, 2005·American Journal of Physiology. Cell Physiology·David M Cohen
Jun 22, 1999·Experimental Eye Research·M MirshahiM K Agarwal
Apr 27, 2004·Experimental Eye Research·Mortimer M Civan, Anthony D C Macknight
Dec 6, 2008·Experimental Eye Research·Chi Wai Do, Mortimer M Civan

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