Reimplantation of the ureter after unilateral ureteral obstruction provides a model that allows functional evaluation

Kidney International
T T TapmeierW Wong

Abstract

Experimental unilateral ureteral obstruction (UUO) is widely used to study renal fibrosis; however, renal injury can only be scored semiobjectively by histology. We sought to improve the UUO model by reimplanting the obstructed ureter followed by removal of the contralateral kidney, thus allowing longitudinal measurements of renal function. Mice underwent UUO for different lengths of time before ureteral reimplantation and contralateral nephrectomy. Measurement of blood urea nitrogen (BUN) allows objective evaluation of residual renal function. Seven weeks after reimplantation and contralateral nephrectomy, mean BUN levels were increased with longer duration of UUO. Interstitial expansion, fibrosis, and T-cell and macrophage infiltration were similar in kidneys harvested after 10 days of UUO or following 10 weeks of ureter reimplantation, suggesting that the inflammatory process persisted despite relief of obstruction. Urinary protein excretion after reimplantation was significantly increased compared to control animals. Our study shows that functional assessment of the formerly obstructed kidney can be made after reimplantation and may provide a useful model to test therapeutic strategies for reversing renal fibrosis and prese...Continue Reading

References

Nov 13, 1998·The New England Journal of Medicine·G Remuzzi, T Bertani
Feb 23, 1999·Kidney International·R L ChevalierJ T Wolstenholme
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Saulo Klahr, Jeremiah Morrissey
Oct 14, 2005·Journal of the American Society of Nephrology : JASN·Anita L CochraneSharon D Ricardo
Jan 18, 2006·Annual Review of Medicine·Raymond C Harris, Eric G Neilson
Aug 31, 2007·Kidney International·B A ThornhillR L Chevalier

❮ Previous
Next ❯

Citations

Jun 23, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Allison A EddyIkuyo Yamaguchi
Jan 22, 2010·American Journal of Physiology. Renal Physiology·Tipu S PuriRichard J Quigg
Feb 16, 2012·International Journal of Nanomedicine·M E M DolmanRobbert J Kok
Apr 3, 2009·Kidney International·Robert L ChevalierBarbara A Thornhill
Mar 16, 2012·Kidney International·Naïke BigeJean-Jacques Boffa
Feb 28, 2009·Kidney International·Jeremiah J Morrissey
Jun 18, 2010·Kidney International·Thomas T TapmeierWilson Wong
Aug 5, 2017·Journal of the American Society of Nephrology : JASN·Ming LiangJizhong Cheng
Jun 20, 2019·Renal Failure·Jie SongWei Zhu
Mar 6, 2021·The American Journal of the Medical Sciences·Jie SongWei Zhu

❮ Previous
Next ❯

Related Concepts

Related Feeds