Reinforced Epithelial Barrier Integrity via Matriptase Induction with Sphingosine-1-Phosphate Did Not Result in Disturbances in Physiological Redox Status

Oxidative Medicine and Cellular Longevity
Erzsebet Paszti-GereA Székács

Abstract

Objectives. The relationship among matriptase function, cellular redox status, and maintenance of intestinal barrier integrity has not been established yet. The aim of this study is to reveal if the crosstalk between matriptase activators and intestinal epithelial monolayers can lead to perturbations in physiological redox regulation in vitro. Methods. The effects of suramin and sphingosine-1-phosphate (S1P) were tested on viability of intestinal porcine epithelial IPEC-J2 cells using MTS assay. Measurements of transepithelial electrical resistance (TER) were performed to determine changes in barrier integrity of cell monolayers. Amplex Red assay was used to monitor extracellular hydrogen peroxide production. Occludin distribution pattern was detected prior to and after matriptase activation using immunofluorescent staining technique. Results. TER reduction was observed in suramin-treated IPEC-J2 cell monolayers, which could be attributed to cell cytotoxic properties of 48 hr 50 μM suramin administration. In contrast, S1P treatment increased TER significantly and elevated occludin accumulation in tight junctions. It was also found that extracellular hydrogen peroxide levels were maintained in IPEC-J2 cells exposed to matriptase...Continue Reading

References

Jan 17, 2002·The Journal of Biological Chemistry·Christelle BenaudChen-Yong Lin
Mar 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kane L SchaphorstJoe G N Garcia
Nov 27, 2009·The Journal of Biological Chemistry·I-Chu TsengChen-Yong Lin
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Marguerite S BuzzaToni M Antalis
May 7, 2010·International Journal of Food Microbiology·Avrelija Cencic, Tomaz Langerholc
Mar 19, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rana Al-SadiThomas Ma
Nov 15, 2011·Veterinary Microbiology·Amanda J Brosnahan, David R Brown
Nov 15, 2011·Inflammatory Bowel Diseases·Sarah Netzel-ArnettToni M Antalis
Jun 30, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Erzsebet Pászti-GereTorsten Steinmetzer

❮ Previous
Next ❯

Citations

Oct 30, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Erzsébet Pászti-GereAndrás Székács
Apr 14, 2017·World Journal of Gastroenterology : WJG·Hanne Van SpaendonkBenedicte Y De Winter
Jun 4, 2018·Brain : a Journal of Neurology·Carlos R Camara-LemarroyV Wee Yong
Dec 18, 2019·Acta Veterinaria Hungarica·Réka Fanni BarnaErzsébet Pásztiné Gere
Nov 17, 2020·The Journal of Investigative Dermatology·Satomi IgawaAnna Di Nardo

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
PCR
Assay
Fluorescence
transfection

Software Mentioned

R
LUCIA Cytogenetics

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Mathias DevreeseSiska Croubels
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Victoria McGilliganIan Rowland
Journal of Enzyme Inhibition and Medicinal Chemistry
Erzsebet Paszti-GereTorsten Steinmetzer
© 2021 Meta ULC. All rights reserved