Reinforcement of the bactericidal effect of ciprofloxacin on Pseudomonas aeruginosa biofilm by hyperbaric oxygen treatment

International Journal of Antimicrobial Agents
Mette KolpenPeter Ø Jensen

Abstract

Chronic Pseudomonas aeruginosa lung infection is the most severe complication in cystic fibrosis patients. It is characterised by antibiotic-tolerant biofilms in the endobronchial mucus with zones of oxygen (O2) depletion mainly due to polymorphonuclear leucocyte activity. Whilst the exact mechanisms affecting antibiotic effectiveness on biofilms remain unclear, accumulating evidence suggests that the efficacy of several bactericidal antibiotics such as ciprofloxacin is enhanced by stimulation of the aerobic respiration of pathogens, and that lack of O2 increases their tolerance. Reoxygenation of O2-depleted biofilms may thus improve susceptibility to ciprofloxacin possibly by restoring aerobic respiration. We tested such a strategy using reoxygenation of O2-depleted P. aeruginosa strain PAO1 agarose-embedded biofilms by hyperbaric oxygen treatment (HBOT) (100% O2, 2.8bar), enhancing the diffusive supply for aerobic respiration during ciprofloxacin treatment. This proof-of-principle study demonstrates that biofilm reoxygenation by HBOT can significantly enhance the bactericidal activity of ciprofloxacin on P. aeruginosa. Combining ciprofloxacin treatment with HBOT thus clearly has potential to improve the treatment of P. aerugi...Continue Reading

References

Feb 6, 2002·The Journal of Clinical Investigation·Dieter WorlitzschGerd Döring
Sep 7, 2007·Cell·Michael A KohanskiJames J Collins
Oct 7, 2009·Expert Review of Anti-infective Therapy·Maide CimşitSenol Yildiz
Dec 8, 2010·Future Microbiology·Niels HøibyThomas Bjarnsholt
Oct 2, 2013·Nature Reviews. Drug Discovery·Thomas BjarnsholtNiels Høiby
Dec 10, 2013·International Journal of Antimicrobial Agents·Rikke Prejh BrochmannPeter Østrup Jensen
Mar 13, 2014·Clinical and Experimental Immunology·M KolpenP Ø Jensen
May 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daniel J DwyerJames J Collins

❮ Previous
Next ❯

Citations

Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Oana CiofuAntonio Oliver
Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Peter Ø JensenMichael Kühl
May 6, 2017·Journal of Wound Care·Finn GottrupPasquale Longobardi
Apr 4, 2018·Stereotactic and Functional Neurosurgery·Jiri BartekGaston Schechtmann
Mar 5, 2017·Applied and Environmental Microbiology·Majken SønderholmThomas Bjarnsholt
Sep 7, 2017·Antimicrobial Agents and Chemotherapy·Mette KolpenPeter Ø Jensen
Nov 20, 2019·Antimicrobial Agents and Chemotherapy·Mette KolpenNiels Høiby
Feb 19, 2020·Journal of Wound Care·Jan StryjaDeborah Xuereb
Mar 24, 2017·FEMS Microbiology Reviews·Diarmaid Hughes, Dan I Andersson
Jan 16, 2018·Journal of Wound Care·Nicholas E SanfordRandall Wolcott
Dec 13, 2017·International Journal of Molecular Sciences·Majken SønderholmKasper N Kragh
Feb 22, 2017·Medical Gas Research·Hu LvWen-Wu Liu
Sep 27, 2019·Frontiers in Microbiology·Lasse KvichThomas Bjarnsholt
Dec 8, 2016·Drug Delivery and Translational Research·Kamal DuaPhilip M Hansbro
Feb 18, 2020·Medical Hypotheses·Tanja BatinacIgor Barković
Nov 7, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad Yousef MemarHossein Bannazadeh Baghi
Oct 20, 2017·Current Opinion in Microbiology·Jason H YangJames J Collins
May 20, 2021·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Morten HedetoftOle Hyldegaard
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Christian Johann LercheClaus Moser
Jun 14, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Franziska A SchwartzClaus Moser
Feb 4, 2019·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Signe Agnete MøllerMette Kolpen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.