Reinterpreting the best biomarker of oxidative stress: The 8-iso-PGF(2α)/PGF(2α) ratio distinguishes chemical from enzymatic lipid peroxidation

Free Radical Biology & Medicine
Thomas J van 't ErveRonald P Mason

Abstract

The biomarker 8-iso-prostaglandin F2α (8-iso-PGF2α) is regarded as the gold standard for detection of excessive chemical lipid peroxidation in humans. However, biosynthesis of 8-iso-PGF2α via enzymatic lipid peroxidation by prostaglandin-endoperoxide synthases (PGHSs), which are significantly induced in inflammation, could lead to incorrect biomarker interpretation. To resolve the ambiguity with this biomarker, the ratio of 8-iso-PGF2α to prostaglandin F2α (PGF2α) is established as a quantitative measure to distinguish enzymatic from chemical lipid peroxidation in vitro, in animal models, and in humans. Using this method, we find that chemical lipid peroxidation contributes only 3% to the total 8-iso-PGF2α in the plasma of rats. In contrast, the 8-iso-PGF2α levels in plasma of human males are generated >99% by chemical lipid peroxidation. This establishes the potential for an alternate pathway of biomarker synthesis, and draws into question the source of increases in 8-iso-PGF2α seen in many human diseases. In conclusion, increases in 8-iso-PGF2α do not necessarily reflect increases in oxidative stress; therefore, past studies using 8-iso-PGF2α as a marker of oxidative stress may have been misinterpreted. The 8-iso-PGF2α/PGF2α ...Continue Reading

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Feb 9, 2016·Free Radical Biology & Medicine·N V MargaritelisM G Nikolaidis
Mar 26, 2016·Free Radical Biology & Medicine·Kristin A GuertinPatricia A Cassano
Dec 19, 2015·Molecular Nutrition & Food Research·Michael A ZulyniakDavid M Mutch
Apr 4, 2017·Redox Biology·James N CobleyGareth W Davison
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May 30, 2021·Prostaglandins & Other Lipid Mediators·Ghassan MustafaKay D Beharry
Feb 23, 2019·Environmental Science & Technology·Thomas J van T ErveKelly K Ferguson

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