Rejection of cardiac allografts by T cells expressing a restricted repertoire of T-cell receptor V beta genes

Immunology
H ShirwanD V Cramer

Abstract

We have recently shown that T cells infiltrating cardiac allografts early in graft rejection use a limited T-cell receptor (TCR) V beta repertoire. In this study we tested whether this limited repertoire of V beta genes is important for graft rejection. A cell line, AL2-L3, was established from LEW lymphocytes infiltrating ACI heart allografts 2 days after transplantation. This cell line is composed of CD4+ T cells that primarily recognize the class II RTI.B major histocompatibility complex (MHC) molecule expressed by the donor graft. This cell line precipitated acute rejection of donor hearts with a median survival time (MST) of 10.5 days following adoptive transfer to sublethally irradiated LEW recipients. This rate of graft rejection was significantly (P < 0.0007) accelerated when compared with a MST of 60 days for allografts in irradiated control recipients. The AL2-L3-mediated acceleration of graft rejection was donor specific as WF third-party heart allografts were rejected with a delayed tempo (MST = 28.5 days). The V beta repertoire of this cell line was primarily restricted to the expression of V beta 4, 15 and 19 genes. The nucleotide sequence analysis of the beta-chain cDNAs from this cell line demonstrated that the ...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S DemotzH M Grey
Jan 1, 1989·The Journal of Experimental Medicine·J BillE Palmer
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J SchneckA Singer
Aug 4, 1988·Nature·M M Davis, P J Bjorkman
Apr 7, 1995·Science·M G McHeyzer-Williams, M M Davis
Jun 1, 1993·The Journal of Experimental Medicine·Z LiuN Suciu-Foca
Mar 31, 1995·Science·G A BentleyR A Mariuzza
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M Connolly
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A GossT H Hansen
Feb 1, 1993·The Journal of Experimental Medicine·G E OsmanL E Hood

❮ Previous
Next ❯

Citations

Mar 27, 2001·Transplantation Proceedings·E YolcuH Shirwan
Oct 26, 1999·Transplantation·P DouillardJ P Soulillou
Mar 12, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Cristiam M AlvarezCaner Süsal
May 20, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Haval ShirwanSherif Ibrahim
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Victoria F MoxhamWilson Wong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.