Rejection of transplanted hearts in patients evaluated by the component analysis of multi-wavelength NAD(P)H fluorescence lifetime spectroscopy

Journal of Biophotonics
Dusan ChorvatAlzbeta Chorvatova

Abstract

Rejection of transplanted hearts remains one of the principal reasons for death of paediatric patients, but an appropriate diagnostic tool for the mild rejection in early stages is still missing. Tissue autofluorescence (AF) is one of the most versatile non-invasive tools for mapping the metabolic state in living tissues. Increasing interest in the imaging and diagnosis of living cells and tissues based on their intrinsic fluorescence rather than fluorescence labelling is closely connected to the latest developments in high-performance spectroscopy and microscopy techniques. In this contribution, we investigate individual components in spectrally- and time-resolved NAD(P)H fluorescence, revealed by linear unmixing, responsible for increased fluorescence in patients presenting mild rejection of transplanted hearts. Application of such approach has the potential to improve the diagnostics of the cardiac transplant rejection by helping currently used histological analysis.

References

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Citations

Aug 1, 2012·Experimental Physiology·F ElzwieiA Chorvatova
Sep 13, 2011·The Canadian Journal of Cardiology·Valerie Bassien-CapsaAlzbeta Chorvatova
Sep 28, 2010·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Herbert Stepp, Ronald Sroka
Sep 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Patrick M SchaeferBjoern von Einem

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