Rejuvenation by shoot apex culture recapitulates the developmental increase of methylation at the maize gene Pl-Blotched.

Plant Molecular Biology
Erin E Irish, Douglas McMurray

Abstract

Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a myb-like transcription factor that regulates anthocyanin production in maize, is one such gene: certain cytosines at the 3' end of this allele are hypomethylated in seedlings, become hypermethylated in organs formed in the adult phase, and are hypomethylated again in the next generation. We tested whether this developmental pattern of low juvenile cytosine methylation followed by higher methylation in adult tissues could also be observed in plants "rejuvenated" via shoot apex culture. We found that cytosine methylation patterns at Pl-Blotched were indeed recapitulated in culture-rejuvenated plants, showing hypomethylation in leaves with juvenile patterns of differentiation (even though they were made by an old meristem) followed by hypermethylation in later-formed leaves. Our results show that methylation status at that locus is determined by the developmental phase of the shoot, rather than by the age of the merist...Continue Reading

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Citations

Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·R S PoethigG Wu
Oct 11, 2008·PLoS Genetics·Jaswinder SinghDamon Lisch
Sep 4, 2008·The Plant Journal : for Cell and Molecular Biology·Josh StrableErin E Irish
Aug 12, 2020·Plant Cell Reports·Zijie ZhangYun Li

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