RelA binding of mRNAs modulates translation or sRNA-mRNA basepairing depending on the position of the GGAG site.

Molecular Microbiology
Pallabi Basu, Shoshy Altuvia

Abstract

Previously, we reported that RelA protein facilitates Hfq-mediated mRNA-sRNA regulation by binding sRNAs carrying a Shine-Dalgarno-like GGAG sequence. In turn, sRNA-Hfq monomers are stabilized, enabling the attachment of more Hfq subunits to form a functional hexamer. Here, using CLIP-seq, we present a global analysis of RelA-bound RNAs showing that RelA interacts with sRNAs as well as with mRNAs carrying a GGAG motif. RelA binding of mRNAs carrying GGAG at position -7 relative to the initiation codon (AUG) inhibits translation by interfering with the binding of 30S ribosomes. The extent of inhibition depends on the distance of GGAG relative to the AUG, as shortening the spacing between GGAG and AUG abrogates RelA-mediated inhibition. Interestingly, RelA binding of target mRNAs carrying GGAG in the coding sequence or close to AUG facilitates target gene regulation by sRNA partners that lack GGAG. However, translation inhibition caused by RelA binding of mRNAs carrying GGAG at position -7 relative to the AUG renders sRNA-mRNA basepairing regulation ineffective. Our study indicates that by binding RNAs carrying GGAG the ribosome-associated RelA protein inhibits translation of specific newly synthesized incoming mRNAs or enables b...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A M FallonM Nomura
Aug 27, 1990·Biochimica Et Biophysica Acta·C PortierC Ehresmann
Jan 20, 1990·Journal of Molecular Biology·C PortierM Grunberg-Manago
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J R Cole, M Nomura
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C PhilippeC Ehresmann
May 31, 1996·Journal of Molecular Biology·K D Murray, H Bremer
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jul 11, 2001·Genes & Development·K M WassarmanS Gottesman
Jun 18, 2002·Molecular Microbiology·Carol S BakerPaul Babitzke
Mar 26, 2003·Nucleic Acids Research·Ruth HershbergHanah Margalit
Dec 20, 2005·Molecular Microbiology·Maude Guillier, Susan Gottesman
Mar 16, 2007·Structure·Tatsuya KaminishiShigeyuki Yokoyama
Mar 27, 2007·Current Opinion in Microbiology·Paul Babitzke, Tony Romeo
Apr 22, 2010·The EMBO Journal·Erik HolmqvistEduard Gerhart Heinrich Wagner
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Toby SoperSarah A Woodson
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Alan G Hinnebusch
Mar 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Liron ArgamanShoshy Altuvia
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Apr 9, 2015·Nature Reviews. Microbiology·Vasili HauryliukKenn Gerdes
Feb 19, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi YamamotoKnud H Nierhaus
May 6, 2016·Molecular Microbiology·Wieland Steinchen, Gert Bange
May 21, 2016·Science·Venu KamarthapuEvgeny Nudler
Jun 16, 2016·Wiley Interdisciplinary Reviews. RNA·Michelle M Meyer
Sep 3, 2016·Molecular Cell·Sahar MelamedHanah Margalit
Sep 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alexandre SmirnovJörg Vogel
Mar 25, 2017·The EMBO Journal·Alexandre SmirnovJörg Vogel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.