PMID: 8474167May 1, 1993Paper

Relating structure to function in the hepatitis delta virus antigen

Journal of Virology
D W Lazinski, J M Taylor

Abstract

Hepatitis delta virus expresses two forms of a single protein, the small (delta Ag-S) and large (delta Ag-L) antigens, which are identical except for an additional 19 residues present at the C terminus of delta Ag-L. While delta Ag-S is required to promote genome replication, delta Ag-L potently inhibits this process and also facilitates packaging of the viral genome by envelope proteins of the helper virus (hepatitis B virus). Regions within the antigens responsible for nuclear localization, RNA binding, and dimerization have been identified, yet it is not clear how these particular activities contribute to the ultimate replication and packaging phenotypes. Here we report the following findings. (i) Although the removal of the nuclear localization signal from either antigen resulted in significant cytoplasmic accumulation, both proteins still had access to the nucleus. As a consequence, no functional defect was observed with either mutant. (ii) The RNA-binding domain, although necessary for delta Ag-S function, could be deleted from delta Ag-L without compromising its ability to either inhibit replication or promote packaging. (iii) In contrast, the coiled-coil dimerization domain was required for both the activation of replic...Continue Reading

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Citations

Apr 14, 2004·Journal of Virology·Xingcao NieJohn M Taylor
Mar 16, 2006·Journal of Virology·Jinhong ChangJohn Taylor
Oct 25, 2016·International Journal of Molecular Sciences·Vera L TunitskayaAlexander V Ivanov
Jul 1, 1996·Journal of Viral Hepatitis·J Monjardino
Oct 29, 2003·Journal of Virology·Jinhong ChangJohn M Taylor
Dec 1, 2009·Viruses·Chung-Hsin Tseng, Michael M C Lai
Mar 29, 2000·Critical Reviews in Clinical Laboratory Sciences·L E Modahl, M M Lai
May 24, 2000·Journal of Virology·G MoraledaJ Taylor
Sep 13, 2003·Journal of Virology·Cromwell T Cornillez-Ty, David W Lazinski
Feb 16, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Natalia FreitasSeverin O Gudima
Apr 1, 1997·Journal of Gastroenterology and Hepatology·S J Hadziyannis
Jul 25, 2000·Journal of Virology·S GudimaJ Taylor
Nov 20, 2018·Médecine sciences : M/S·Natali Abeywickrama-SamarakoonPaul Dény
Sep 12, 2000·Journal of Virology·J ChangJ Taylor
Mar 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sheng-Chieh HsuJaw-Ching Wu
Dec 20, 2018·Viruses·Michelle WilleAeron C Hurt
May 30, 1998·Journal of Virology·K DingleJ Taylor
Aug 10, 1999·Journal of Virology·G MoraledaJ Taylor
Mar 1, 1996·The Journal of Biological Chemistry·J C Otto, P J Casey
Sep 1, 1996·Journal of Virology·V V BichkoJ M Taylor

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