PMID: 8954136Nov 22, 1996Paper

Relation between accumulation of phospholipase A2 reaction products and Ca2+ release in isolated liver mitochondria

Biochimica Et Biophysica Acta
I RustenbeckS Lenzen

Abstract

A Ca(2+)-dependent stimulation of mitochondrial phospholipase A2 is often assumed to play a role in mitochondrial Ca2+ release. We sought to clarify this relation by measuring Ca2+ transport and determining phospholipase A2 reaction products from the same sample of isolated, incubated rat liver mitochondria. When mitochondria had accumulated and spontaneously released again Ca2+, most probably by membrane permeability transition, there was no increase of phospholipase A2 reaction products. However, when the incubation was continued after Ca2+ release, significant increases of the content of lysophosphatidylcholine and unesterified fatty acids could be seen. Quinacrine, an inhibitor of phospholipase A2 activity, prevented Ca2+ release and p-hydroxymercuribenzoic acid, an inhibitor of lysophospholipid reesterification, induced a fast release of Ca2+ from isolated mitochondria. Such effects are usually taken as indirect evidence for a participation of phospholipase A2 in mitochondrial Ca2+ release, but analysis of the mitochondrial lipids revealed that no significant changes of the mass of phospholipase A2 reaction products had occurred. These experiments suggest that the accumulation of phospholipase A2 reaction products in mitoc...Continue Reading

References

Jul 1, 1979·Archives of Biochemistry and Biophysics·R A Haworth, D R Hunter
Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Mar 1, 1978·Archives of Biochemistry and Biophysics·A M SechiG Lenaz
May 2, 1978·Biochemistry·J W ParceM Waite
Sep 1, 1992·The Biochemical Journal·S LenzenI Rustenbeck
Oct 14, 1991·Biochimica Et Biophysica Acta·I RustenbeckS Lenzen
Oct 1, 1990·Biochemical Society Transactions·N YafeiM Crompton
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Nov 15, 1985·Archives of Biochemistry and Biophysics·R S Cockrell
Jul 1, 1989·Trends in Pharmacological Sciences·S OrreniusP Nicotera
Aug 30, 1989·Biochemical and Biophysical Research Communications·K M Broekemeier, D R Pfeiffer
Feb 1, 1985·Archives of Biochemistry and Biophysics·T OkayasuJ L Farber
Sep 1, 1985·Analytical Biochemistry·S Lenzen, U Panten
Jun 15, 1980·The Biochemical Journal·J W ParceC C Cunningham
Aug 1, 1994·The American Journal of Physiology·T E GunterC E Gavin
Aug 16, 1993·Biochemical and Biophysical Research Communications·I RustenbeckS Lenzen
May 1, 1993·Biochemical Society Transactions·A P HalestrapC P Connern
Feb 5, 1996·Journal of Biochemical and Biophysical Methods·I Rustenbeck, S Lenzen

❮ Previous
Next ❯

Citations

Sep 17, 2005·Journal of Bioenergetics and Biomembranes·Christos ChinopoulosGary Fiskum
Jan 18, 2003·Biochimica Et Biophysica Acta·Jeanie B McMillin, William Dowhan
Apr 16, 1998·British Journal of Pharmacology·T Grimmsmann, I Rustenbeck
Jan 31, 2008·Journal of Clinical Biochemistry and Nutrition·Teruo UmegakiJunzo Sasaki
Mar 30, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jason L BlumRick G Schnellmann
Oct 30, 2009·Journal of Cellular and Molecular Medicine·Claudia PiccoliNazzareno Capitanio
Jun 10, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Noemí GarcíaEdmundo Chávez
Sep 9, 2017·Cell Biology International·Mabel Buelna-ChontalEdmundo Chávez
Jul 3, 2004·The Journal of Biological Chemistry·Lara MacchioniGianfrancesco Goracci

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.