PMID: 9540843Apr 16, 1998Paper

Relation between enzyme release and irreversible cell injury of the heart under the influence of cytoskeleton modulating agents

Biochimica Et Biophysica Acta
E Skobel, H Kammermeier

Abstract

The effects of agents modulating the cytoskeleton, taxol (microtubuli stabilizing), vinblastine (microtubuli destabilizing) and cytochalasin D (actin destabilizing) (10(-6) M each) on enzyme and ATP release as well as on irreversible cell injury were investigated in isolated perfused hypoxic and reoxygenated rat hearts. Enzyme (creatine kinase (CK)) and ATP concentration were assayed in the interstitial transudate and venous effluent. Irreversible cell injury was determined from trypan blue uptake and nuclear staining (NS) of cardiomyocytes in histologic sections. ATP release from nonneuronal cells was only detectable in the interstitial transudate and was not significantly altered by the agents. In controls total CK release (about 4% of total CK) exceeded the percentage of irreversibly injured cells by a factor of 8. Taxol and cytochalasin D abolished the hypoxia/reoxygenation induced interstitial CK release and reduced total CK release to a highly significant extent. The percentage of irreversible injured cells was even more diminished by these agents resulting in a ratio of CK/NS of 40. The effect of cytochalasin D apparently is the consequence of decreased contractile performance as shown by analogous depression by butonedi...Continue Reading

Citations

Dec 4, 2003·Progress in Biophysics and Molecular Biology·S C CalaghanE White
Nov 17, 2009·Cellular and Molecular Life Sciences : CMLS·Jiong-Yu HuYue-Sheng Huang
Jul 31, 2007·Journal of Molecular and Cellular Cardiology·Kanu ChatterjeeJoel S Karliner
Jun 22, 2010·Journal of Cellular and Molecular Medicine·Junjie XiaoYi-Han Chen
Jun 11, 2002·Archives of Biochemistry and Biophysics·Min Suk KimInsug Kang
Feb 1, 2005·Regulatory Peptides·Chunhua CaoSuhn Hee Kim
Feb 28, 2019·American Journal of Physiology. Cell Physiology·Kati MadoTuuli Kaambre
Jan 5, 1999·The Journal of Biological Chemistry·Y FischerC Löken
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Naoya OketaniChuwa Tei

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