PMID: 4886045Apr 1, 1969Paper

Relation between Epstein-Barr viral and cell membrane immunofluorescence in Burkitt tumor cells. IV. Differentiation between antibodies responsible for membrane and viral immunofluorescence

The Journal of Experimental Medicine
G PearsonP Clifford

Abstract

Previous reports (1, 2) have established that the expression of certain distinctive membrane antigen(s) on the surface of Burkitt's lymphoma (BL) and infectious mononucleosis (IM) cells is dependent on the presence of Epstein-Barr virus (EBV) in the cell line. The investigations reported here provide evidence that antibodies directed against EBV antigens, as revealed by the immunofluorescence test on acetone-fixed smears (8), and the membrane reactive antibodies, although often present in the same serum, are nevertheless distinctly different. Absorption of Mutua serum, the standard reference serum for demonstrating membrane antigen(s) on BL and IM cells, with BL cells completely removed anti-membrane activity without significantly affecting the anti-EBV antibody titer. Furthermore, sera were found which contained one type of antibody but not the other. Sera with high anti-membrane but low anti-EBV activity were found among relatives of BL patients. These sera reacted with the membranes of EBV-carrying BL and IM cells in essentially the same way, i.e., against the same spectrum of target cells, as the EBV-positive Mutua serum. They were unable to block the membrane reactivity of FITC-conjugated Mutua serum, however. In some case...Continue Reading

Citations

Aug 7, 1969·The New England Journal of Medicine·R S Schwartz
Aug 1, 1971·Australasian Radiology·D S Nelson, M Nelson
Jun 21, 1971·Annals of the New York Academy of Sciences·A SvedmyrP Clifford
Jun 21, 1971·Annals of the New York Academy of Sciences·A KawamuraM Takada
Nov 22, 1969·British Medical Journal
Mar 1, 1971·Postgraduate Medical Journal·G Klein
Oct 1, 1970·The Journal of Surgical Research·L J Humphrey
Jan 15, 1976·International Journal of Cancer. Journal International Du Cancer·A de SchryverG Klein
Jul 15, 1970·International Journal of Cancer. Journal International Du Cancer·J S NadkarniP Clifford
May 15, 1970·International Journal of Cancer. Journal International Du Cancer·P Clifford
Nov 15, 1969·International Journal of Cancer. Journal International Du Cancer·J Yata, G Klein
Jul 15, 1969·International Journal of Cancer. Journal International Du Cancer·G KleinL J Old
Nov 15, 1971·International Journal of Cancer. Journal International Du Cancer·B JohanssonG Henle
Sep 15, 1971·International Journal of Cancer. Journal International Du Cancer·G HenleG Klein
Mar 15, 1974·International Journal of Cancer. Journal International Du Cancer·A de SchryverG Henle
Sep 15, 1971·International Journal of Cancer. Journal International Du Cancer·D SilvestreJ P Levy
Nov 15, 1970·International Journal of Cancer. Journal International Du Cancer·B JohanssonG Henle
Jul 15, 1971·International Journal of Cancer. Journal International Du Cancer·M K Walters, J H Pope
Oct 1, 1978·Journal of Virology·G R Pearson, L F Qualtiere

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.