Relation Between Mitochondrial Membrane Potential and ROS Formation

Methods in Molecular Biology
Jan M SuskiMariusz R Wieckowski

Abstract

Mitochondria are considered the main source of reactive oxygen species (ROS) in the cell. For this reason they have been recognized as a source of various pathological conditions as well as aging. Chronic increase in the rate of ROS production is responsible for the accumulation of ROS-associated damages in DNA, proteins, and lipids and may result in progressive cell dysfunctions and, in a consequence, apoptosis, increasing the overall probability of an organism's pathological conditions. The superoxide anion is the main undesired by-product of mitochondrial oxidative phosphorylation. Its production is triggered by a leak of electrons from the mitochondrial respiratory chain and the reaction of these electrons with O2. Superoxide dismutase (MnSOD, SOD2) from the mitochondrial matrix, as well as superoxide dismutase (Cu/ZnSOD, SOD1) present in small amounts in the mitochondrial intramembrane space, converts superoxide anion to hydrogen peroxide, which can be then converted by catalase to harmless H2O.In the chapter we describe a relation between mitochondrial membrane potential and the rate of ROS formation. We present different methods applicable for isolated mitochondria or intact cells. We also present experiments demonstrati...Continue Reading

Citations

Nov 30, 2019·Frontiers in Pharmacology·Natalia Pavlovna AkentievaSergei Michailovich Aldoshin
Nov 27, 2019·Chemical Research in Toxicology·Santosh Kumar Palanirajan, Sathyanarayana N Gummadi
Dec 18, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Joanna KopeckaChiara Riganti
Jan 26, 2021·Biochemistry Research International·Edmundo ChávezNatalia Pavón
Sep 10, 2020·Burns : Journal of the International Society for Burn Injuries·Wenli ZhaoLingling Liu
May 26, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Alp Özgün, Bora Garipcan
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