Abstract
Aminoglycoside antibiotics exhibit a markedly reduced antibacterial activity under anaerobic conditions. Anaerobiosis or inhibitors of electron transport produced an extensive decrease in the uptake of dihydrostreptomycin in Escherichia coli K-12. Uptake of proline or putrescine were only slightly impaired under anaerobic conditions in the presence of glucose. Both the susceptibility to and the uptake of dihydrostreptomycin under anaerobic conditions were partially restored by addition of the alternative electron acceptor, nitrate. This stimulation required functional nitrate reductase activity. Abolition of uptake by 2,4-dinitrophenol under both aerobic and anaerobic conditions indicates that streptomycin uptake requires electron transport as well as a sufficient membrane potential. In addition, the initial rate of dihydrostreptomycin uptake was competitively and reversibly inhibited by added salts. The inhibition was relatively nonspecific with respect to the identity of salt added, being approximately dependent on the ionic strength. Although dihydrostreptomycin and polyamines mutually inhibited each other's uptake, several conditions (polyamine limitation, streptomycin uptake-deficient mutants) were found in which uptake of...Continue Reading
References
Oct 1, 1978·Antimicrobial Agents and Chemotherapy·P DickieM A Pickard
Sep 1, 1975·Journal of Bacteriology·R J Kadner, H H Winkler
Jan 1, 1976·Journal of Supramolecular Structure·V BraunA Hartmann
Aug 1, 1977·Antimicrobial Agents and Chemotherapy·L E Bryan, H M Van Den Elzen
May 16, 1978·European Journal of Biochemistry·J V Höltje
Jan 1, 1979·Antimicrobial Agents and Chemotherapy·L E BryanH M Van Den Elzen
Jan 1, 1978·Annual Review of Biochemistry·J M DiRienzoM Inouye
Sep 1, 1978·Archives of Biochemistry and Biophysics·W W Kay, M Cameron
Feb 1, 1979·Antimicrobial Agents and Chemotherapy·J V Höltje
Dec 1, 1978·Antimicrobial Agents and Chemotherapy·L J Harrell, J B Evans
Jun 1, 1976·Antimicrobial Agents and Chemotherapy·L E Bryan, H M Van den Elzen
Jul 1, 1956·Journal of General Microbiology·G M WILLIAMSON, F WHITE
Citations
Dec 1, 1981·Antimicrobial Agents and Chemotherapy·P D Damper, W Epstein
Apr 1, 1982·Antimicrobial Agents and Chemotherapy·S J KohlheppD N Gilbert
Apr 1, 1983·Antimicrobial Agents and Chemotherapy·S M MatesM H Miller
Jun 1, 1983·Antimicrobial Agents and Chemotherapy·L E Bryan, S Kwan
Oct 1, 1984·Antimicrobial Agents and Chemotherapy·A S McEnroe, H W Taber
Jan 1, 1986·Antimicrobial Agents and Chemotherapy·A S Arrow, H W Taber
Dec 1, 1986·Antimicrobial Agents and Chemotherapy·J M ZenilmanL J Mandel
Feb 1, 1990·Antimicrobial Agents and Chemotherapy·A CavalleroG C Schito
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·W MaoO Lomovskaya
Feb 29, 2000·Journal of Bacteriology·E Y RosenbergH Nikaido
Jan 2, 2010·BMC Microbiology·Rachel Binet, Anthony T Maurelli
Jun 6, 2006·Advances in Carbohydrate Chemistry and Biochemistry·Bert Willis, Dev P Arya
Aug 15, 1989·Analytical Biochemistry·W S Champney
Jan 1, 1987·Biochimica Et Biophysica Acta·W W Nichols
Apr 25, 2018·Nature Communications·Erik Wistrand-YuenDan I Andersson
Jan 9, 1998·Journal of Bacteriology·D R MacingaP N Rather
Oct 31, 2020·PLoS Pathogens·Michael M Maiden, Christopher M Waters
Jun 23, 2020·ACS Pharmacology & Translational Science·Bikash Baral, M R Mozafari
Apr 29, 1998·Antimicrobial Agents and Chemotherapy·M R ParadiseP N Rather
Dec 1, 1987·Microbiological Reviews·H W TaberA S Arrow