PMID: 15228095Jul 2, 2004Paper

Relations of the type and branch of surface N-glycans to cell adhesion, migration and integrin expressions

Molecular and Cellular Biochemistry
Y ZhangHui-Li Chen

Abstract

The relations between the structure of cell surface N-glycans to cell behaviors were studied in H7721 human hepatocarcinoma cell line, which predominantly expressed complex-type N-glycans on the surface. 1-Deoxymannojirimycin (DMJ) and swaisonine (SW), the specific inhibitor of Golgi alpha-mannosidase II or I, were selected to block the processing of N-glycans at the steps of high mannose and hybrid type respectively. All-trans retinoic acid (ATRA) and antisense cDNA of N-acetylglucosaminyltransferase-V (GnT-V) were used to suppress the expression of GnT-V and decreased the GlcNAc beta1,6-branching or tri-/tetra-antennary structure of surface N-glycans. The structural alterations of N-glycans were verified by sequential lectin affinity chromatography of [3H] mannose-labeled glycans isolated from the cell surface. The cell adhesions to fibronectin (Fn) and human umbilical vein epithelial cell (HUVEC), as well as cell migration (including chemotaxis and invasion) were selected as the parameters of cell behaviors. It was found that cell adhesion and migration were significantly decreased in SW and DMJ treated cells, suggesting that complex type N-glycan is critical for the above cell behaviors. ATRA and antisense GnTV enhanced cel...Continue Reading

Citations

Sep 5, 2015·Analytical Cellular Pathology (Amsterdam)·Ewa PochećAnna Lityńska
May 3, 2008·Journal of Cellular Biochemistry·Qiu-Yan WangHui-Li Chen
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Seon-Joo YoonSen-itiroh Hakomori
Mar 18, 2008·Cellular and Molecular Neurobiology·Xiaoyun YangMeiyu Geng
Nov 26, 2010·Cellular and Molecular Life Sciences : CMLS·Salomé S PinhoCelso A Reis
Apr 2, 2014·Cell Biology International·Marcelina Elżbieta JanikMałgorzata Przybyło
Aug 15, 2008·Cancer Research·Reza Beheshti ZavarehAaron D Schimmer
Feb 14, 2021·Reproduction in Domestic Animals = Zuchthygiene·Monika JamiołMarta Kankofer
Dec 6, 2011·Nano Letters·Andreas MaderMadeleine Leisner
Mar 25, 2010·Biochimica Et Biophysica Acta·Marcelina E JanikPierre Vereecken
May 14, 2020·Oncology Research and Treatment·Chenglong LiYuan Ruan

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