Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates.

Journal of Clinical Microbiology
P Di MartinoA Darfeuille-Michaud

Abstract

Klebsiella pneumoniae is an opportunistic gram-negative pathogen involved in outbreaks of nosocomial infections in intensive care units. Strains are resistant to multiple antibiotics, and 15 to 30% of them are also resistant to the broad-spectrum cephalosporins by the production of R plasmid-encoded extended-spectrum beta-lactamases. Because the gastrointestinal tracts of patients have been shown to be the reservoir for nosocomial strains of K. pneumoniae, we looked for a correlation between antibiotic resistance and adhesion of K. pneumoniae strains to intestinal cells. We investigated adhesion to the human intestinal epithelial Caco-2 cell line of 61 clinical K. pneumoniae strains isolated in hospitals in Clermont-Ferrand, France. None of the strains tested expressed the previously described adhesive factors CF29K and KPF-28. Adhesive properties were found for 42.6% of the strains tested (26 strains). Just 7.7% (2 strains) of the 26 strains producing only the chromosomally encoded SHV-1 beta-lactamase adhered to the Caco-2 cell line, whereas 68.5% (24 strains) of the 35 strains producing a plasmid-encoded beta-lactamase were adherent. All the adherent strains, and even the two strains producing only the SHV-1 enzyme, harbored...Continue Reading

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Citations

Aug 16, 2008·The Journal of Antimicrobial Chemotherapy·Susanne SchjørringKaren A Krogfelt
Dec 5, 2009·Archives of Microbiology·Aleksander Deptuła, Eugenia Gospodarek
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·H SahlyU Ullmann
Jan 20, 2009·Journal of Bacteriology·Steen G StahlhutEvgeni V Sokurenko
Apr 13, 2000·Journal of Bacteriology·M Espinosa-UrgelJ L Ramos
Feb 10, 2004·Applied and Environmental Microbiology·Artashes R KhachatryanDouglas R Call
May 1, 2021·International Journal of Molecular Sciences·Vanessa AratoFrancesca Micoli

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