Relationship between Anti-Spike Protein Antibody Titers and SARS-CoV-2 In Vitro Virus Neutralization in Convalescent Plasma

BioRxiv : the Preprint Server for Biology
Eric SalazarJames M Musser

Abstract

Newly emerged pathogens such as SARS-CoV-2 highlight the urgent need for assays that detect levels of neutralizing antibodies that may be protective. We studied the relationship between anti-spike ectodomain (ECD) and anti-receptor binding domain (RBD) IgG titers, and SARS-CoV-2 virus neutralization (VN) titers generated by two different in vitro assays using convalescent plasma samples obtained from 68 COVID-19 patients, including 13 who donated plasma multiple times. Only 23% (16/68) of donors had been hospitalized. We also studied 16 samples from subjects found to have anti-spike protein IgG during surveillance screening of asymptomatic individuals. We report a strong positive correlation between both plasma anti-RBD and anti-ECD IgG titers, and in vitro VN titer. Anti-RBD plasma IgG correlated slightly better than anti-ECD IgG titer with VN titer. The probability of a VN titer ≥160 was 80% or greater with anti-RBD or anti-ECD titers of ≥1:1350. Thirty-seven percent (25/68) of convalescent plasma donors lacked VN titers ≥160, the FDA-recommended level for convalescent plasma used for COVID-19 treatment. Dyspnea, hospitalization, and disease severity were significantly associated with higher VN titer. Frequent donation of con...Continue Reading

Citations

Aug 15, 2020·Clinical Microbiology Reviews·Daniele FocosiMarco Tuccori
Sep 10, 2020·International Journal of Molecular Sciences·Neelu BatraAi-Ming Yu
Jan 26, 2021·Immunologic Research·Phani Kumar DevarasettiYerram Keerthi Vardhan
Jan 16, 2021·Proceedings of the National Academy of Sciences of the United States of America·Varun SasisekharanUma Narayanasami
Dec 12, 2020·Scientific Reports·John V DzimianskiRebecca M DuBois
Jan 10, 2021·Journal of Clinical Microbiology·Nathalie RenardFranck Berthier
Apr 8, 2021·JMIR Public Health and Surveillance·Henry T PengAndrew Beckett
Apr 24, 2021·Microbial Cell Factories·Norma A Valdez-CruzMauricio A Trujillo-Roldán

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Datasets Mentioned

BETA
MN908947

Methods Mentioned

BETA
ELISA
ELISAs
bronchoalveolar lavage
Assay

Software Mentioned

R
RAxML
7500 SDS
CLC Genomics Workbench
Harmonia

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