Relationship between antigenicity and pathogenicity for Burkholderia pseudomallei and Burkholderia mallei revealed by a large panel of mouse MAbs

Hybridoma
Nianxiang ZouShyh-Ching Lo

Abstract

Burkholderia pseudomallei and Burkholderia mallei are two closely related gram-negative bacterial species classified by the CDC as category B biowarfare agents. To develop monoclonal antibodies (MAbs) that can recognize as many different strains and/or clinical isolates of these two pathogens as possible, we immunized mice with heat-killed bacterial whole cells and membrane preparations from multiple strains and/or clinical isolates of B. pseudomallei and B. mallei. More than 100 different hybridoma clones that produced MAbs strongly reacting to B. pseudomallei and/or B. mallei have been developed. These MAbs were categorized into eight different groups according to their reaction specificity against different species of Burkholderia bacteria as well as the different nature of target antigens (LPS, capsule polysaccharides, proteins, and glycoproteins) on the bacteria they recognized. Characterization of this large panel of MAbs has demonstrated an interesting pattern of various antigenic epitopes shared by the different species of Burkholderia bacteria. More importantly, this study has revealed a pathogenicity-linked antigen epitope(s) on capsule-like polysaccharides found only in the pathogenic species of Burkholderia bacteria...Continue Reading

References

May 1, 1989·Reviews of Infectious Diseases·A Leelarasamee, S Bovornkitti
May 1, 1989·The Journal of Infectious Diseases·W ChaowagulN Pitakwatchara
Nov 1, 1995·Chest·M IpT A Raffin
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jul 19, 2002·Molecular Microbiology·Inga Benz, M Alexander Schmidt
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·William C NiermanClaire M Fraser
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew T G HoldenJulian Parkhill
Jan 1, 1947·Annals of Internal Medicine·C HOWE, W R MILLER

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Citations

May 24, 2011·Journal of Bacteriology·Andrew E ScottJoann L Prior
Jun 22, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Pengfei GuoShyh-Ching Lo
Oct 20, 2017·Journal of Medical Microbiology·Shirley Yi Fen HiiFairuz Amran
Mar 29, 2014·Clinical and Vaccine Immunology : CVI·Wildaliz NievesLisa A Morici
Feb 9, 2021·Frontiers in Microbiology·Chalita ChomkatekaewClaire Chewapreecha

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