Relationship between asparagine metabolism and protein concentration in soybean seed.

Journal of Experimental Botany
Sudhakar PanduranganFrédéric Marsolais

Abstract

The relationship between asparagine metabolism and protein concentration was investigated in soybean seed. Phenotyping of a population of recombinant inbred lines adapted to Illinois confirmed a positive correlation between free asparagine levels in developing seeds and protein concentration at maturity. Analysis of a second population of recombinant inbred lines adapted to Ontario associated the elevated free asparagine trait with two of four quantitative trait loci determining population variation for protein concentration, including a major one on chromosome 20 (linkage group I) which has been reported in multiple populations. In the seed coat, levels of asparagine synthetase were high at 50 mg and progressively declined until 150 mg seed weight, suggesting that nitrogenous assimilates are pre-conditioned at early developmental stages to enable a high concentration of asparagine in the embryo. The levels of asparaginase B1 showed an opposite pattern, being low at 50 mg and progressively increased until 150 mg, coinciding with an active phase of storage reserve accumulation. In a pair of genetically related cultivars, ∼2-fold higher levels of asparaginase B1 protein and activity in seed coat, were associated with high protein...Continue Reading

References

Aug 1, 1991·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P C FrancisN V Owen
Aug 1, 1995·Genome Research·W J PavanS M Tilghman
Jan 1, 1997·Plant Molecular Biology·C A HughesB F Matthews
Jan 1, 1984·Botanical Gazette·C D RaperR J Downs
Jun 14, 2003·Plant Physiology·Hon-Ming LamGloria M Coruzzi
Dec 10, 2003·Genome Génome / Conseil National De Recherches Canada·Stephen J MolnarElroy R Cober
Jun 16, 2004·Current Opinion in Cell Biology·Lijun Sun, Zhijian J Chen
Sep 7, 2004·Plant Physiology·Sari A RuuskaJohn B Ohlrogge
Sep 18, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Isao KuboKen-ichi Nihei
Apr 2, 2008·Current Opinion in Biotechnology·Jörg Schwender
Apr 23, 2008·Current Protocols in Protein Science·Tong Sun, Ralph B Arlinghaus
Dec 17, 2008·The Plant Journal : for Cell and Molecular Biology·Doug K AllenYair Shachar-Hill
Apr 28, 2009·The Plant Journal : for Cell and Molecular Biology·Ann SandersMechthild Tegeder
Dec 18, 2009·Planta·Jinxin YiSangeeta Dhaubhadel
Jan 16, 2010·Nature·Jeremy SchmutzScott A Jackson
Nov 18, 2010·Molecular Plant·Mechthild Tegeder, Doris Rentsch
Feb 25, 2011·Journal of Agricultural and Food Chemistry·Hari B Krishnan, Randall L Nelson
Jun 22, 2011·Plant, Cell & Environment·Elizabeth A AinsworthStephen P Long
Mar 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B W DiersR C Shoemaker

❮ Previous
Next ❯

Citations

Jul 13, 2013·Journal of Plant Physiology·Gregorio Gálvez-ValdiviesoManuel Pineda
Nov 20, 2012·Plant & Cell Physiology·Alfredo CredaliAntonio J Márquez
Jun 22, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dan ZhangDeyue Yu
Jun 21, 2014·Journal of Experimental Botany·Marco BettiAntonio J Márquez
Apr 12, 2016·Frontiers in Plant Science·Sudhakar PanduranganFrédéric Marsolais
Apr 23, 2015·Physiologia Plantarum·Sudhakar PanduranganFrédéric Marsolais
Feb 25, 2015·BMC Genomics·Ling LiEve Syrkin Wurtele
Mar 10, 2015·Frontiers in Plant Science·Sudhakar PanduranganFrédéric Marsolais
Sep 19, 2014·Frontiers in Plant Science·Eliot M Herman
Sep 28, 2016·PeerJ·Steven C HuberIvan Baxter
Jun 20, 2019·The Plant Journal : for Cell and Molecular Biology·Jaya JoshiFrédéric Marsolais
Sep 3, 2018·Molecular Genetics and Genomics : MGG·Jimmy ObalaRajeev K Varshney
Apr 9, 2017·The Plant Journal : for Cell and Molecular Biology·Laure GaufichonAkira Suzuki
Jan 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiyu LiHailong Ning
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder
Jun 7, 2013·Journal of Agricultural and Food Chemistry·Thushan S Withana-GamageJanitha P D Wanasundara

❮ Previous
Next ❯

Datasets Mentioned

BETA
D28123
Glyma18g02060

Methods Mentioned

BETA
transgenic
Protein Assay
affinity purification
electrophoresis
PCR
reverse transcription-PCR

Software Mentioned

Primer
SAS
MapMaker
Quantity One
MQTL
Genemed
Excel
SuperANOVA
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.