Relationship between cellular diadenosine 5',5'''-P1,P4-tetraphosphate level, cell density, cell growth stimulation and toxic stresses

Experimental Cell Research
E Segal, J B Le Pecq

Abstract

In order to elucidate the postulated role of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cell growth regulation, the Ap4A cellular content was measured in cells submitted to various treatments affecting the cell growth. Ap4A level was found to increase ten times when cells reached confluence, whereas no significant variation of the ATP pool was observed. Cell growth arrest after serum depletion did not cause any variation in the Ap4A pool. A limited increase in the Ap4A pool was observed when growth of arrested cells was reinitiated but this variation reflected only the increase of cell density. No significant variation in the Ap4A intracellular level was observed after submitting two eukaryotic cell lines to various stresses (cytotoxic drugs, ethanol and heat-shock treatments). These results suggest that, in eukaryotic cells, Ap4A is not involved in cell growth stimulation but rather is associated with cell contact growth inhibition. They also suggest that Ap4A is not an 'alarmone', contrary to what has been proposed for bacteria.

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Grummt
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F GrummtC C Kuenzle
Jul 6, 1966·Biochemical and Biophysical Research Communications·P C ZamecnikK Randerath
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P C LeeB N Ames
Nov 15, 1984·Biochemical Pharmacology·J P BendirdjianA Jacquemin-Sablon
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P C ZamecnikE F Baril

❮ Previous
Next ❯

Citations

Jan 1, 1989·The International Journal of Biochemistry·M Andersson
Jan 1, 1990·The International Journal of Biochemistry·M AnderssonH Bergstrand
Jun 8, 2002·Journal of Molecular Biology·Stephen G Addinall, Barry Holland
Sep 29, 2000·Pharmacology & Therapeutics·A G McLennan
Jan 15, 1988·European Journal of Biochemistry·A M CourgeonM Best-Belpomme
Jun 10, 2010·International Journal of Cancer. Journal International Du Cancer·Kimako OkaHironobu Sasano
Jun 1, 1990·Journal of Biochemical and Biophysical Methods·M J CostasJ C Cameselle
Jun 1, 1993·The Journal of Investigative Dermatology·A ReanoD Schmitt
Mar 20, 1990·Journal of Molecular Biology·F LévĕqueS Blanquet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.