Relationship between changes in mRNAs of the genes encoding steroidogenic acute regulatory protein and P450 cholesterol side chain cleavage in head kidney and plasma levels of cortisol in response to different kinds of acute stress in the rainbow trout (Oncorhynchus mykiss)

General and Comparative Endocrinology
Malika Geslin, Benoit Auperin

Abstract

In this study, the expression of several genes involved in cortisol synthesis in head kidneys, the site of cortisol production, and in the rainbow trout (Oncorhynchus mykiss) was examined in response to two different acute stressors and an acute ACTH treatment. mRNAs levels of the "steroidogenic acute regulatory" (StAR) sterol transport protein, which transports cholesterol to the inner mitochondrial membrane as well as cytochrome P450 cholesterol side chain cleavage (P450(SCC)) were determined in head kidney (containing the interrenal tissue). In one experiment, we also quantified 3-beta-hydroxysteroid dehydrogenase (3B-HSD) and cytochrome P450(11beta) (11B-H) mRNAs. The presence of these four transcripts in the head kidney was confirmed by Northern blot analysis. For each stress condition, mRNA levels were quantified by quantitative or real-time RT-PCR. The results of these two methods were highly correlated. An acute stress induced by capture, short confinement (2min), and anesthesia (3min) resulted in significant elevation of plasma cortisol (30-fold higher than controls) and an increase in levels of StAR and P450(SCC) mRNAs 3h post-stress. When fish were submitted to an acute stress caused by 5min of chase with a net in a ...Continue Reading

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Citations

Nov 26, 2013·General and Comparative Endocrinology·J D JeffreyK M Gilmour
Feb 3, 2009·Journal of Toxicology and Environmental Health. Part a·Trond M KortnerAugustine Arukwe
Apr 18, 2013·Zebrafish·Anusha K S DhanasiriViswanath Kiron
Sep 27, 2008·Pakistan Journal of Biological Sciences : PJBS·Abbasi FatemehJamili Shahla
Feb 14, 2012·General and Comparative Endocrinology·Jennifer D JeffreyKathleen M Gilmour
Sep 25, 2007·Aquatic Toxicology·Amélie Gravel, Mathilakath M Vijayan
Sep 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Trond M KortnerAugustine Arukwe
Apr 20, 2017·The Journal of Endocrinology·Ana Patrícia MateusDeborah M Power
Mar 23, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Amélie Gravel, Mathilakath M Vijayan
Dec 19, 2018·Frontiers in Endocrinology·Nataly SanhuezaSebastian Boltana
Nov 5, 2019·General and Comparative Endocrinology·Melinda S PruchaNancy D Denslow
Jun 10, 2015·The Journal of Experimental Biology·Angelico MadaroMarnix Gorissen
Oct 19, 2012·The Journal of Experimental Biology·Marta Conde-SieiraJosé L Soengas
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Jan 20, 2007·Physiology & Behavior·Pierre MormèdeIsabelle Veissier
Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry
Mar 20, 2022·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Madison L EarhartPatricia M Schulte

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