PMID: 8592198Nov 1, 1995Paper

Relationship between changes of glomus cell current and neural response of rat carotid body

Journal of Neurophysiology
P M Cheng, D F Donnelly

Abstract

1. Mature rat carotid bodies were harvested and sinus nerve activity was recorded in vitro during superfusion with Ringer saline. Membrane currents of glomus cells were simultaneously recorded using conventional whole cell or perforated-patch whole cell recording. Presumptive glomus cells were identified by the presence of a rapidly activated, voltage-dependent outward current above a threshold of -20 mV. 2. Outward current of presumptive glomus cells was inhibited by tetraethylammonium chloride (TEA) (20 mM) and by verapamil (5-10 microM), consistent with previous studies in which isolated glomus cells were used. Somal capacitance, calculated from the current transient following a step hyperpolarization, was 7.47 +/- 0.54 (SE) pF (n = 52). Membrane resistance for perforated-patch recordings was 820 +/- 187 M omega. 3. In perforated-patch recordings, brief periods of hypoxia (30-45 s) caused a marked increase in nerve activity to 21.6 +/- 2.7 times baseline spiking frequency (n = 59) but no significant change in membrane resistance or outward current. No change in holding current was detected, although the low amplifier gain precluded high-resolution measurement. Similar results were obtained using conventional whole cell recor...Continue Reading

Citations

Jan 1, 1997·Annual Review of Physiology·G G Haddad, C Jiang
May 25, 2002·Acta Anaesthesiologica Scandinavica·M JonssonL I Eriksson
Aug 1, 1997·Experimental Neurology·A S ChidekelG G Haddad
Feb 10, 2006·Respiratory Physiology & Neurobiology·M J WasickoJ L Carroll
Apr 10, 2007·Respiratory Physiology & Neurobiology·Keith J Buckler

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