Relationship between chaperone activity and oligomeric size of recombinant human alphaA- and alphaB-crystallin: a tryptic digestion study

Proteins
S Saha, K P Das

Abstract

Alpha-crystallin, the major eye lens protein, exists as a large oligomer of two subunits, alphaA- and alphaB-crystallin. The individual subunits assemble into the oligomer in vitro. It is generally believed that oligomerization is pre-requisite for chaperone function, although there is no hard data available on this subject. We therefore undertook a study using limited tryptic digestion as a tool for examining the relationship between oligomeric size and chaperone activity of recombinant alphaA- and alphaB-crystallin. We showed that tryptic digested fragments of both alphaA- and alphaB-crystallin much smaller than the original subunits retain considerable chaperone activity. Our results indicate that chaperone activity depends more on the sequence of the reduced peptide than on its oligomeric size. The results also suggest that the presence of the alpha-crystallin domain and hydrophobic clefts on the protein surface, which correlate poorly with oligomeric size, are important for chaperone function.

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Citations

May 24, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·André-Patrick Arrigo, Benjamin Gibert
Sep 4, 2015·Chemical Communications : Chem Comm·Maya A WrightTuomas P J Knowles
Jan 9, 2007·Biochemical and Biophysical Research Communications·J Andrew Aquilina, Stephen J Watt
Nov 1, 2006·Protein Science : a Publication of the Protein Society·Puttur Santhoshkumar, K Krishna Sharma
Feb 17, 2006·Analytical Biochemistry·Lingyun LiuShaoyi Jiang
Feb 28, 2015·International Journal of Biological Macromolecules·Srinivasagan RamkumarKumarasamy Anbarasu
Nov 29, 2011·Biochimica Et Biophysica Acta·Ram H NagarajAshis Biswas
Apr 4, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Victor Banerjee, K P Das
Aug 14, 2012·Archives of Toxicology·Daniel R CioccaStuart K Calderwood
May 16, 2018·Biochemistry·Maya A WrightTuomas P J Knowles

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