Relationship between conformation of polysaccharides -in the dilute regime and their interaction with a phospholipid bilayer

Luminescence : the Journal of Biological and Chemical Luminescence
S GirodJ M Devoisselle

Abstract

Interactions between polysaccharides and phospholipid bilayers have already been demonstrated in the literature but little is known about the influence of macromolecule conformations related to the solvent characteristics (pH, ions, ionic strength). In this study we have investigated the conformation of iono- and thermo-sensitive polysaccharides, iota- and kappa-carrageenans, and their interaction with a dimyristoylphosphatidylcholine (DMPC) model bilayer. The study was performed in two different media (NaCl 150 mmol/L, pH 6.5, and NaCl 300 mmol/L, pH 6.5). In the first part, the iota- and kappa-carrageenan samples have been characterized by size exclusion chromatography (SEC) coupled with a multi-angle laser light-scattering detector (MALLS). The SEC-MALLS results clearly show polysaccharide chain association at high ionic strength. In the second part, the polysaccharide-membrane interaction has been studied, using fluorescent probes embedded in the membrane. The thermotropic properties of the membrane were investigated by fluorescence depolarization of 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). The membrane surface accessibility was evaluated by fluorescence quenching of 2-(9-anthroyloxy) stearic acid...Continue Reading

References

Apr 1, 1990·Chemistry and Physics of Lipids·M Krumbiegel, K Arnold
Mar 18, 1991·Biochimica Et Biophysica Acta·S CohenR Langer
Sep 1, 1990·Chemistry and Physics of Lipids·K ArnoldM Krumbiegel
Apr 1, 1996·International Journal of Biological Macromolecules·B DenefH Reynaers
Oct 1, 1996·International Journal of Biological Macromolecules·N MischenkoH Reynaers
Jul 1, 1997·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·M Mobed, T M Chang

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Citations

Jul 26, 2006·World Journal of Gastroenterology : WJG·Ricardo Vázquez-RamírezRolando Hernández-Muñoz
May 7, 2009·The Journal of Physical Chemistry. B·Thatyane M NobreMaria Elisabete D Zaniquelli

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