Relationship between CYP1A enzyme activities and protein levels in rats treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin

Journal of Toxicology and Environmental Health
M J DeVitoL S Birnbaum

Abstract

Induction of CYP1A1 is one of the best characterized responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). EROD activity has been used as an enzymatic marker for CYP1A1 following TCDD treatment. Enzymatic markers for the induction of CYP1A2 by TCDD are not as well characterized. The present study examines the relationship between CYP1A1 and CYP1A2 protein and the corresponding enzymatic markers. Induction of hepatic ethoxyresorufin O-deethylase (EROD) activity and methoxyresorufin O-demethylase (MEROD) and acetanilide 4-hydroxylase (ACOH) activity (both markers for CYP1A2) were analyzed in 8-wk-old male and female Fischer 344 rats treated orally with either 0, 0.1, 0.3, 1.0, or 3.0 micrograms TCDD/kg. There were no sex differences in basal EROD or ACOH activity. MEROD activity was significantly greater in control males than in control females. Significant induction of EROD activity in females occurred at slightly lower doses of TCDD compared to males (0.1 vs. 0.3 micrograms/kg, respectively); however, a greater absolute and a larger fold induction of EROD activity was seen in males compared to females at all doses tested except 0.1 micrograms/kg. EROD activity did not attain a maximum in either sex. Similarly, MEROD activity...Continue Reading

Citations

Dec 23, 2003·Aquatic Toxicology·Jeanmarie M ZodrowRobert L Tanguay
Jul 4, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D E AmacherJ E Burkhardt
Oct 25, 2002·Journal of Toxicology and Environmental Health. Part a·Hui-Wu WangTzuu-Huei Ueng
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Jul 26, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Janice S LeeJ Christopher Corton
Feb 11, 1999·Biochemical Pharmacology·J T HammL S Birnbaum
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Jul 21, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stephen J GodinMatthew K Ross
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Aug 18, 2020·Regulatory Toxicology and Pharmacology : RTP·Christian StruppPramila Singh
Apr 7, 1997·Biochemical and Biophysical Research Communications·M J SantostefanoL S Birnbaum

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