PMID: 15374447Mar 1, 1991Paper

Relationship between enzymatic activity loss and post-translational protein modification in aging

Archives of Gerontology and Geriatrics
A MachadoC Santa Maria

Abstract

During aging, there is a decrease in the activity of many enzymes. The mechanism causing the loss of activity is still not well understood in most cases. We have studied the decrease in the activities of the malic, 6-phosphogluconate dehydrogenase and superoxide dismutase enzymes. The old malic enzyme is about 36% less active than the young enzyme and the old 6-phosphogluconic dehydrogenase enzyme is about 26% less active than the young enzyme. In this paper, some chemical properties of these enzymes are studied. Diethyl pyrocarbonate measurements indicate that the old malic enzyme has 1 histidine residue less than the young malic enzyme. Moreover, the treatment of the young malic enzyme with ascorbate for 15 min produces the loss of 36% of enzymatic activity and the loss of 1.2 histidine residues. 2,4,6-trinitrobenzenesulfonic acid measurements indicate that the old 6-phosphogluconate dehydrogenase enzyme has 11 lysine residues less than the young 6-phosphogluconate dehydrogenase enzyme. The proteolysis with trypsin produces more peptides in the young 6-phosphogluconate dehydrogenase enzyme than in the old one. However, similar numbers of peptides were produced when endoproteinase Arg-C was used in both enzymes, young and old ...Continue Reading

References

Apr 1, 1976·European Journal of Biochemistry·U Reiss, D Gershon
Dec 1, 1979·Mechanisms of Ageing and Development·A Z Reznick, D Gershon
May 1, 1976·Archives of Biochemistry and Biophysics·H K SharmaM Rothstein
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Feb 14, 1980·Biochimica Et Biophysica Acta·G G Chang, T M Huang

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Citations

Oct 4, 1996·Mechanisms of Ageing and Development·F DíazI Pérez de Vargas
Mar 1, 1994·Neurobiology of Aging·T FunahashiJ M Carney
Jan 1, 1996·Free Radical Biology & Medicine·C P De La CruzA Machado
Apr 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sethumadhavan SavithaChinnakannu Panneerselvam
Apr 11, 2001·Experimental Gerontology·R A FloydK Hensley
Nov 6, 2010·Antioxidants & Redox Signaling·Dongping Liu, Yang Xu
Apr 1, 1992·Brain Pathology·J Müller-Höcker
Nov 1, 1996·Free Radical Research·C Santa MaríaE Revilla

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