Relationship between expression of 5-fluorouracil metabolic enzymes and 5-fluorouracil sensitivity in esophageal carcinoma cell lines

Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus
T AndoY Fujii

Abstract

5-Fluorouracil (5-FU) is a key drug in the treatment of esophageal squamous cell carcinoma (ESCC). Gene expression of 5-FU metabolic enzymes such as thymidylate synthase (TS), thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD) and orotate phosphoribosyl transferase (OPRT), has recently been investigated in order to predict the 5-FU sensitivity of several cancers. We examined the relationship between such gene expression and 5-FU sensitivity in 25 ESCC cell lines. TS, DPD, TP and OPRT mRNA levels were assessed by real-time polymerase chain reaction. The 50% inhibitory concentrations (IC50) of 5-FU in 25 ESCC cell lines were determined by cell proliferation assay. IC50 values for 5-FU ranged from 1.00 to 39.81 micromol/L. There were significant positive correlations between IC50 and TS mRNA expression (R(2) = 0.5781, P < 0.0001) and DPD mRNA expression (R(2) = 0.3573, P = 0.0016). There were no correlations between IC50 and TP or OPRT mRNA expression. TS and DPD mRNA expression levels may be useful indicators in predicting the anti-tumor activity of 5-FU in ESCC.

References

Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A BeckG Milano
Oct 23, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C G LeichmanP V Danenberg
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C AscheleA Sobrero
Mar 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David EdlerHenric Blomgren
Mar 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Carmen J AllegraRichard M Goldberg
Jun 27, 2002·Biochimica Et Biophysica Acta·G J PetersH M Pinedo
Dec 24, 2002·Japanese Journal of Cancer Research : Gann·Hisataka FujiwaraMasakazu Fukushima
May 2, 2003·Nature Reviews. Cancer·Daniel B LongleyPatrick G Johnston
May 31, 2003·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Masanori TerashimaMitsukazu Gotoh
Apr 3, 2004·Cancer Chemotherapy and Pharmacology·Mikito InokuchiKenichi Sugihara
Jul 13, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yasushi ShintaniHikaru Matsuda

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Citations

Aug 23, 2012·World Journal of Gastroenterology : WJG·Chariya HahnvajanawongWichittra Tassaneeyakul
May 12, 2016·Biotechnology Letters·Xiao WanZhanfeng Cui
Apr 8, 2016·Clinical and Translational Medicine·Björn L D M BrücherIjaz S Jamall
Dec 14, 2011·Cellular and Molecular Life Sciences : CMLS·Sheru Sanbhnani, Foong May Yeong
Apr 29, 2010·Journal of Cancer Research and Clinical Oncology·Dimitrios J StravopodisGerassimos E Voutsinas
Sep 10, 2019·Journal of Labelled Compounds & Radiopharmaceuticals·Derya Ilem-OzdemirMakbule Asikoglu
Jul 7, 2012·International Journal of Oncology·Tetsuya TamataniYouji Miyamoto
Mar 1, 2011·Oncology Letters·Masahiro KimuraHiromitsu Takeyama
Apr 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kevin DzoboCollet Dandara

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