PMID: 8592168Jan 1, 1995Paper

Relationship between expression of a major apurinic/apyrimidinic endonuclease (APEX nuclease) and susceptibility to genotoxic agents in human glioma cell lines

Journal of Neuro-oncology
Y OnoS Seki

Abstract

The multifunctional DNA repair enzyme (APEX nuclease) having apurinic/apyrimidinic (AP) endonuclease, 3'-5' exonuclease, DNA 3' repair diesterase and DNA 3'-phosphatase activities is thought to be involved in repair of AP sites and single-strand breaks with 3'-blocked termini. To investigate the biological role of the enzyme, we studied the correlation between APEX AP endonuclease activity in several human glioma cell lines having various degree of its expression and cellular susceptibility to cytotoxic agents such as methyl methanesulfonate (MMS), 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3- (2-chloroethyl)-3-nitrosourea hydrochloride (ACNU), cis-diamminedichloroplatinum(II) (CDDP), etoposide (VP-16), hydrogen peroxide (H2O2), hyperthermia and X-ray. The cell lines having lower APEX expression showed higher sensitivity to MMS and H2O2 which are known to induce AP sites and single strand breaks on DNA, respectively. The cellular susceptibility to the other agents tested was not significantly correlated to the APEX expression. The present results are thought to support the notion that APEX nuclease plays an important role on repair of AP sites and single-strand DNA breaks with 3'-blocked termini in mammalian cells.

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Citations

Oct 6, 2000·Mutation Research·A R EvansM R Kelley
Nov 4, 2008·Antioxidants & Redox Signaling·Gianluca TellMark R Kelley
Sep 6, 2005·The Journal of Gene Medicine·Catherine C SmithNigel J Gooderham
Jan 25, 2011·Journal of Applied Toxicology : JAT·Moacir de Azevedo Bentes Monteiro NetoDenise Crispim Tavares
Aug 2, 2006·Mutation Research·Melissa L FishelMark R Kelley
Jun 11, 1999·Biochemical and Biophysical Research Communications·L Zhu, B D Halligan
Nov 17, 2009·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Mi-Young KangMi-Hwa Kim
Apr 24, 2004·The Cochrane Database of Systematic Reviews·H Powell, P G Gibson
Aug 4, 2010·Journal of Radiation Research·Mamta D NaiduLouis A Peña

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