Relationship between gyrA mutations and quinolone resistance in Flavobacterium psychrophilum isolates

Applied and Environmental Microbiology
S Izumi, F Aranishi

Abstract

Flavobacterium psychrophilum is the causative agent of the fish diseases called bacterial cold-water disease and rainbow trout fry syndrome. It has been reported that some isolates of F. psychrophilum are resistant to quinolones; however, the mechanism of this quinolone resistance has been unexplained. In this study, we examined the quinolone susceptibility patterns of 27 F. psychrophilum strains isolated in Japan and the United States. Out of 27 isolates, 14 were resistant to both nalidixic acid (NA) and oxolinic acid (OXA), and the others were susceptible to NA and OXA. When amino acid sequences deduced from gyrA nucleotide sequences of all isolates tested were analyzed, two amino acid substitutions (a threonine residue replaced by an alanine or isoleucine residue in position 83 of GyrA [Escherichia coli numbering] and an aspartic acid residue replaced by a tyrosine residue in position 87) were observed in the 14 quinolone-resistant isolates. These results strongly suggest that, as in other gram-negative bacteria, DNA gyrase is an important target for quinolones in F. psychrophilum.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A SuginoN R Cozzarelli
Jun 1, 1990·Antimicrobial Agents and Chemotherapy·H YoshidaS Nakamura
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·M MaurinD Raoult
Jan 9, 2004·Applied and Environmental Microbiology·Alicia GibelloLucas Domínguez

❮ Previous
Next ❯

Citations

Apr 15, 2011·Journal of Fish Diseases·K Sundell, T Wiklund
May 29, 2013·Environmental Microbiology·Felipe C CabelloAlejandro H Buschmann
Jun 26, 2010·Journal of Aquatic Animal Health·Shohreh HesamiJohn S Lumsden
Oct 24, 2008·Journal of Aquatic Animal Health·Paola NavarreteJaime Romero
Jun 30, 2009·Veterinary Research·Sarah E ClarkFrank A Fekete
Mar 3, 2020·Environmental Microbiology·Yingbo ShenYang Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Autoimmune Hemolytic Anemia

Autoimmune hemolytic anemia (AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to an insufficient number of oxygen-carrying red blood cells in the circulation. Discover the latest research on AIHA here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.