Relationship between impaired apoptosis of lymphocytes and distribution of dendritic cells in peripheral blood and synovial fluid of children with juvenile idiopathic arthritis.

Archivum Immunologiae Et Therapiae Experimentalis
E SmolewskaJerzy Stanczyk

Abstract

The pathogenesis of juvenile idiopathic arthritis (JIA) is not fully understood. Recently the present authors described disturbed apoptosis of JIA lymphocytes in both peripheral blood (PB) and synovial fluid (SF) as well as an abnormal distribution of blood dendritic cells (BDCs) between the PB and SF in this disease. Possible relationships between these events during the development of JIA process are assessed here. Lymphocyte apoptosis and BDC counts were assessed in the PB and SF of untreated JIA children. Lymphocyte apoptosis was analyzed by the Annexin-V/propydium iodide assay. Total DC (TDC) number was based on the sum of three BDC subpopulations determined using a panel of monoclonal antibodies against BDC antigens (BDCA): myeloid type 1 (mDC1, BDCA-1(+)/HLA-DR(+)/CD19(-)), myeloid type 2 (mDC2, BDCA-3(+)/HLA-DR(+)/CD14(-)), and plasmacytoid (pDC, BDCA-2(+)/HLA-DR(+)/CD123(+)). Cells were enumerated by the flow cytometric "single-platform" method. The concentration of tumor necrosis factor (TNF)-alpha and the distribution of particular lymphocyte subtypes in both PB and SF were also investigated. There was significant positive correlation between apoptosis of PB lymphocytes and SF TDC count (p=0.002) as well as SF TNF-al...Continue Reading

References

Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A DzionekJ Schmitz
Mar 20, 2001·Clinical Rheumatology·M YilmazB Antmen
May 16, 2002·Arthritis Research·Antony P B BlackTaunton R Southwood
Oct 24, 2002·Blood·Kelli P A MacDonaldDerek N J Hart
Jul 16, 2003·Annals of the Rheumatic Diseases·E SmolewskaJ Stanczyk
May 6, 2004·Scandinavian Journal of Rheumatology·E SmolewskaJ Stańczyk
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ismé M de KleerBerent Prakken
Jul 1, 2004·Arthritis Research & Therapy·Duojia CaoVivianne Malmström
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberto LandeEliana M Coccia
Dec 4, 2004·American Journal of Respiratory Cell and Molecular Biology·Ingel K DemedtsRomain A Pauwels
Mar 4, 2005·Arthritis Research & Therapy·Lois L CavanaghRanjeny Thomas
Mar 4, 2005·Biology of Reproduction·Arlen SoghomoniansGordon C Douglas
May 19, 2005·Arthritis Research & Therapy·Jan LeipeHendrik Schulze-Koops
Nov 24, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H HacksteinR Weimer
Apr 22, 2006·American Journal of Respiratory Cell and Molecular Biology·Ingel K DemedtsGuy G Brusselle
Sep 14, 2007·Arthritis Research & Therapy·Viviana LutzkyRanjeny Thomas

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Citations

Dec 8, 2014·Best Practice & Research. Clinical Rheumatology·J F Swart, N M Wulffraat

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Methods Mentioned

BETA
ESR
biopsy
flow cytometry

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis