PMID: 9156Oct 5, 1976

Relationship between medium pH and that of the lysosomal matrix as studied by two independent methods

Biochimica Et Biophysica Acta
D J ReijngoudJ M Tager

Abstract

1. The method of estimating the intralysosomal pH by measuring the distribution of [14C]methylamine in lysosomes isolated from the livers of Triton WR 1339-treated rats has been critically examined. 2. In lysed lysosomes, methylamine is bound to the membrane fragments, but this binding can be completely suppressed by increasing the concentration of monovalent cations in the medium. 3. In intact lysosomes, the binding of [14C]methylamine is only partly inhibited by monovalent cations at 25 degrees C. 4. THe accumulation of [14C]methylamine in intact lysosomes is progressively inhibited as the concentration of methylamine is increased. A similar inhibition of [14C]methylamine accumulation is obtained with NH4Cl. 5. Similar values for the intralysosomal pH were obtained from measurements of the distribution of methylamine, dimethylamine and trimethylamine, which are accumulated in the lysosomes, and of 5,5-dimethyloxazolidinedione-2,4, which is excluded. 6. The breakdown of endocytosed 123I-labelled bovine serum albumin by intact isolated lysosomes is much less sensitive to the pH of the medium than the breakdown of added protein by lysed lysosomes. 7. The intralysosomal pH has been estimated by comparing the rate of breakdown of ...Continue Reading

References

Apr 15, 1976·FEBS Letters·D J Reijngoud, J M Tager
Jun 1, 1975·FEBS Letters·D J Reijngoud, J M Tager
Sep 2, 1975·Biochemical and Biophysical Research Communications·P O Seglen
Jun 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·G L Mandell
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S O LieE F Neufeld
Jan 24, 1973·Biochimica Et Biophysica Acta·D J Riejngoud, J M Tager
Jul 1, 1973·FEBS Letters·R Goldman, H Rottenberg

Citations

Jan 1, 1989·The International Journal of Biochemistry·P C Calder, R Geddes
Jan 1, 1990·The International Journal of Biochemistry·P C Calder, R Geddes
Nov 7, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A O GamerJ Heliwig
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T Hackstadt, J C Williams
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Desautels, A L Goldberg
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·A M CantinP Larivée
Jun 1, 1984·The Proceedings of the Nutrition Society·G E Mortimore, C A Surmacz
Apr 19, 1982·FEBS Letters·L J DebeerM J Geelen
Mar 20, 1981·Biochimica Et Biophysica Acta·E H Morgan
Dec 1, 1981·Archives of Biochemistry and Biophysics·J Velema, J Zaagsma
Sep 1, 1978·Experimental Cell Research·J S AmentaS Brocher
Oct 5, 1976·Biochimica Et Biophysica Acta·D J ReijngoudJ M Tager
Dec 1, 1981·The Journal of Experimental Zoology·A ArilloP Mensi
Jan 15, 1979·Biochemical and Biophysical Research Communications·P Dell'Antone
Jul 3, 1980·Nature·A H GordonM R Young
Aug 1, 1984·Journal of Cellular Physiology·S PatersonE H Morgan
Sep 1, 1983·European Journal of Biochemistry·V FlockerziF Hofmann
Nov 15, 1997·The American Journal of Physiology·R B WeinsteinM E Tischler
Jan 24, 2019·Journal of Inherited Metabolic Disease·Leandro R Soria, Nicola Brunetti-Pierri

Related Concepts

Ammonium Chloride
Metazoa
Cations, Monovalent
Dimethadione
Dimethylamines
Hydrogen-Ion Concentration
Liver
Lysosomes
Osmitrol
Tissue Membrane

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