PMID: 6974268Jan 1, 1981Paper

Relationship between membrane potential and calcium ion fluxes in the fragmented sarcoplasmic reticulum

The Japanese Journal of Physiology
M Koshita, K Hotta

Abstract

The relation between membrane potential and Ca2+ fluxes in the skeletal fragmented sarcoplasmic reticulum (FSR) was investigated using cyanine dye (diS-C3-(5) as a potential probe. Change of membrane potential and Ca2+ release from FSR were induced by changing the ionic composition of the medium. When the medium was changed from K-gluconate to Tris-gluconate, a large fluorescence intensity change was observed, while no Ca2+ release occurred. On the other hand, when the medium was changed from K-gluconate to KCl, Ca2+ release occurred notwithstanding a relatively small fluorescence intensity change. This KCl-induced Ca2+ release from FSR was inhibited by the addition of sucrose. Also, the presence of both permeable anion and cation was required for induction of Ca2+ release. These results suggest that there is no correlation between "depolarization" and Ca2+ release. Ca2+ uptake by FSR created the positive-inside potential which was reversed by the application of Ca2+ ionophore A23187. The addition of valinomycin or nigericin, and the replacement of Cl- with gluconate-affected potential formation. These facts suggest that Ca2+ uptake is accompanied by K+ efflux and/or Cl- influx for partial compensation of the change.

Citations

Apr 1, 1989·Journal of Bioenergetics and Biomembranes·P PaladeG Hals

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.