PMID: 11315257Apr 24, 2001Paper

Relationship between metastasis-associated phenotypes and N-glycan structure of surface glycoproteins in human hepatocarcinoma cells

Journal of Cancer Research and Clinical Oncology
H B GuoH L Chen

Abstract

To study the relation of N-glycan structure on cell surface glycoproteins to the metastatic phenotypes. Two human hepatocarcinoma 7721 cell lines transfected with sense or antisense cDNA of GnT-V, named GnT-V/7721 and GnT-V-AS/7721, respectively, were adopted, because GnT-V can change the antennary number and the content of the beta 1,6 GlcNAc branch in N-glycans. The effects of over- and under-expression of GnT-V on the metastasis-associated phenotype of the transfected cells were investigated and compared with the cells mock-transfected with the plasmid vector. In GnT-V/7721 cells, GnT-V activity was increased by 92% compared with the mock cells. HRP-labeled lectin staining of transfected cells showed elevated intensity with HRP-L-PHA and reduced intensity with HRP-ConA, suggesting the increased antennary number and content of the beta 1,6 GlcNAc branch in N-glycans. Analysis of the N-glycan structure of [3H]-labeled glycopeptides prepared from cell-surface [3H] glycoproteins using DSA-affinity chromatography also revealed the above change of the N-glycan structure in a more quantitative manner. GnT-V/7721 cells showed a suppressed cell attachment to fibronectin (Fn) or laminin (Ln), and increased cell migration and invasion ...Continue Reading

Citations

Oct 3, 2003·Biochemical and Biophysical Research Communications·Peng GuoHui-li Chen
Apr 4, 2006·Molecular and Cellular Biology·Annick LaganaIvan R Nabi
Jan 9, 2016·Cancer Investigation·Ewa PochećAnna Lityńska
Apr 28, 2012·Experimental and Molecular Pathology·Ting WeiJian Zhang
Dec 18, 2013·Proteomics·Maja N ChristiansenNicolle H Packer
Oct 30, 2012·The International Journal of Biochemistry & Cell Biology·Rui GuoLi Jia
Dec 5, 2003·Biochimica Et Biophysica Acta·Ewa PochećAnnarita Casbarra
May 21, 2016·Scientific Reports·Jahanshah Ashkani, Kevin J Naidoo
Dec 21, 2017·World Journal of Surgical Oncology·Yong Keun ParkHee-Jung Wang

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