Abstract
To study the relation of N-glycan structure on cell surface glycoproteins to the metastatic phenotypes. Two human hepatocarcinoma 7721 cell lines transfected with sense or antisense cDNA of GnT-V, named GnT-V/7721 and GnT-V-AS/7721, respectively, were adopted, because GnT-V can change the antennary number and the content of the beta 1,6 GlcNAc branch in N-glycans. The effects of over- and under-expression of GnT-V on the metastasis-associated phenotype of the transfected cells were investigated and compared with the cells mock-transfected with the plasmid vector. In GnT-V/7721 cells, GnT-V activity was increased by 92% compared with the mock cells. HRP-labeled lectin staining of transfected cells showed elevated intensity with HRP-L-PHA and reduced intensity with HRP-ConA, suggesting the increased antennary number and content of the beta 1,6 GlcNAc branch in N-glycans. Analysis of the N-glycan structure of [3H]-labeled glycopeptides prepared from cell-surface [3H] glycoproteins using DSA-affinity chromatography also revealed the above change of the N-glycan structure in a more quantitative manner. GnT-V/7721 cells showed a suppressed cell attachment to fibronectin (Fn) or laminin (Ln), and increased cell migration and invasion ...Continue Reading
Citations
Oct 3, 2003·Biochemical and Biophysical Research Communications·Peng GuoHui-li Chen
Apr 4, 2006·Molecular and Cellular Biology·Annick LaganaIvan R Nabi
Jul 11, 2013·PloS One·Zhaohai ZhangLi Jia
Jan 9, 2016·Cancer Investigation·Ewa PochećAnna Lityńska
Apr 28, 2012·Experimental and Molecular Pathology·Ting WeiJian Zhang
Aug 23, 2006·Journal of Cellular Biochemistry·Liying WangXiliang Zha
Dec 18, 2013·Proteomics·Maja N ChristiansenNicolle H Packer
Jun 10, 2015·Japanese Journal of Clinical Oncology·Haiou LiuJiejie Xu
Feb 11, 2015·Placenta·W-C LiaoM-K Shyu
Oct 30, 2012·The International Journal of Biochemistry & Cell Biology·Rui GuoLi Jia
Apr 8, 2015·Breast Cancer Research : BCR·Shu-Min ZhouSheng-Ce Tao
Dec 5, 2003·Biochimica Et Biophysica Acta·Ewa PochećAnnarita Casbarra
May 21, 2016·Scientific Reports·Jahanshah Ashkani, Kevin J Naidoo
Mar 27, 2004·FEBS Letters·Peng GuoHui-li Chen
Dec 21, 2017·World Journal of Surgical Oncology·Yong Keun ParkHee-Jung Wang
Oct 17, 2003·The Journal of Biological Chemistry·Hua-Bei GuoMichael Pierce
Jan 18, 2006·Molecular and Cellular Biochemistry·Peng GuoHui-Li Chen
Dec 4, 2019·Oncology Letters·Sung Kyu SongYongkeun Park
Sep 25, 2020·Clinical Proteomics·Junjie ZhaoJianxin Gu
Jun 3, 2005·Cancer Research·Eric C SealesSusan L Bellis
Feb 7, 2009·Journal of Proteome Research·Jing CaoPengyuan Yang
Apr 17, 2013·Journal of Proteome Research·Hiroyuki KajiHisashi Narimatsu