Relationship between mitochondrial dysfunction and toxicity of propyl gallate in isolated rat hepatocytes

Toxicology
Y NakagawaG A Moore

Abstract

The relationship between cytotoxicity and mitochondrial dysfunction caused by propyl gallate (PG) has been studied in hepatocytes freshly prepared from fasted rats. Hepatocytes isolated from fasted (18 h) rats were significantly more susceptible to the toxicity of PG than hepatocytes from fed rats. The addition of fructose (15 mM), an alternative carbohydrate source, to hepatocyte suspensions resulted in the prevention of PG (1 mM)-induced cell killing accompanied by decrease in intracellular ATP loss during a 3 h-incubation period. Despite this, fructose did not completely prevent an abrupt loss of intracellular glutathione caused by PG, but effectively inhibited the loss of protein thiol levels. Fructose elicited a concentration (0.5-20mM)-dependent protection against the cytotoxicity of 1.5 mM PG. The incubation of hepatocytes with sodium azide (4 mM), an inhibitor of oxidative phosphorylation, enhanced the toxicity induced by PG (1 mM), but coincubation with fructose delayed the onset of toxicity. Neither azide alone nor fructose plus azide did affect the cell viability during the incubation period. Furthermore, the addition of 2 mM salicylamide, nontoxic to hepatocytes during the incubation period, enhanced PG (1 mM)-induc...Continue Reading

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Citations

Feb 17, 2001·Journal of Applied Toxicology : JAT·A M Sciuto, T S Moran
Mar 26, 2003·European Journal of Pharmacology·Sangeeta KhareJaime F Modiano
Dec 10, 2014·Journal of Biochemical and Molecular Toxicology·Gabrielle Jacklin ElerAdelar Bracht
Jul 21, 2009·Chemico-biological Interactions·Gabrielle Jacklin ElerAdelar Bracht
May 19, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christian FreyJorge Ferreira
Feb 7, 2006·Free Radical Biology & Medicine·Giuseppe GalatiPeter J O'Brien
May 3, 2011·Toxicon : Official Journal of the International Society on Toxinology·Marcos A MaioliFábio E Mingatto
Sep 10, 2013·Toxicology and Applied Pharmacology·Gabrielle Jacklin ElerAdelar Bracht
Feb 19, 2008·International Journal of Toxicology
Apr 20, 2018·Future Medicinal Chemistry·Weimei ShiLonghuo Wu

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