PMID: 11928929Apr 4, 2002Paper

Relationship between motility and membrane integrity of boar spermatozoa in media varying in osmolality

Reproduction in Domestic Animals = Zuchthygiene
L FraserJ Strzezek

Abstract

A study was conducted to evaluate the relationship between boar sperm motility and membrane integrity following exposure to media with 150-1120 mOsm. Total sperm motility was defined as the percentage of spermatozoa that had any form of motility was subjectively assessed under a light microscope. Sperm cell damage was expressed as a loss of membrane integrity as measured by a combination of fluorescent stains, carboxyfluorescein diacetate (CFDA) and propidium iodide (PI), and Hoechst 33258 (H33258). There were no significant differences between sperm motility and membrane-intact spermatozoa. as measured by CFDA-PI and H33258, in media with 250 and 300 mOsm. In anisosmotic conditions, a higher amount of membrane-intact spermatozoa than motile spermatozoa was observed. In hypo-osmotic conditions (150 mOsm), a high proportion of spermatozoa had curled or coiled tails and most of them retained their entire membrane integrity, as detected by CFDA-PI. In media with 350-1120 mOsm, some spermatozoa accumulated PI in the head region and CFDA in the mid-piece. These spermatozoa fluoresced blue at the lower region of the head, as detected by H33258. The ATP content in spermatozoa exposed to hypo- and hyperosmotic conditions was markedly r...Continue Reading

References

Jul 1, 1992·Journal of Reproduction and Fertility·E A McLaughlinM G Hull
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Citations

Apr 12, 2011·Asian Journal of Andrology·Md Sharoare HossainHeriberto Rodriguez-Martinez
Oct 31, 2007·Asian Journal of Andrology·Fernando J Pena
Apr 27, 2012·Animal Reproduction Science·B Macías-GarcíaJ M Morrell
Mar 17, 2010·Animal Reproduction Science·Marc YesteSergi Bonet
Jan 18, 2008·Reproduction in Domestic Animals = Zuchthygiene·E BussalleuS Bonet
Jun 12, 2012·Reproduction in Domestic Animals = Zuchthygiene·J Gómez-FernándezE de Mercado
Apr 14, 2005·Theriogenology·Elizabeth BreiningerMartha T Beconi
Feb 18, 2010·Acta Veterinaria Hungarica·Dariusz GaczarzewiczMaria Laszczyńska
Apr 23, 2017·Animal Reproduction Science·J L YánizP Santolaria
Feb 7, 2018·Reproduction in Domestic Animals = Zuchthygiene·A PinyopumminP Wattananit
Mar 9, 2018·Reproduction, Fertility, and Development·J L YánizC Soler
Jan 1, 2013·Polish Journal of Veterinary Sciences·W KordanM Koziorowska-Gilun
Jul 26, 2018·Reproduction in Domestic Animals = Zuchthygiene·Anne Hege Alm-KristiansenElisabeth Kommisrud
Jan 3, 2019·Biopreservation and Biobanking·Luana G P BezerraAlexandre R Silva
Dec 12, 2018·Reproduction in Domestic Animals = Zuchthygiene·Felipe PezoRaúl Segundo Sánchez
Apr 4, 2017·Journal of Veterinary Research·Anna DziekońskaWładysław Kordan
Jun 10, 2021·Reproduction in Domestic Animals = Zuchthygiene·Kayode Babatunde Balogun, Kara Roski Stewart

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