Relationship between myoplasmic calcium transients and calcium currents in frog skeletal muscle.

The Journal of General Physiology
J GarcíaE Stefani

Abstract

Ca2+ currents (ICa) and myoplasmic Ca2+ transients were simultaneously recorded in single muscle fibers from the semitendinosus muscle of Rana pipiens. The vaseline-gap voltage-clamp technique was used. Ca2+ transients were recorded with the metallochromic indicator dye antipyrylazo III. Ca2+ transients consisted of an early fast rising phase followed by a late slower one. The second phase was increased by experimental maneuvers that enlarged ICa, such as augmenting [Ca2+]o (from 2 to 10 mM) or adding (-)-Bay K 8644 (2 microM). When [Ca2+]o was increased, the second phase of the Ca2+ transients and ICa showed an average increase at 0 mV of 2 +/- 0.9 microM (4) and 1.4 +/- 0.3 mA/ml (4), respectively. (-)-Bay K 8644 increased the late phase of the Ca2+ transients and ICa at 0 mV by 0.8 +/- 0.3 microM (3) and 6.7 +/- 2.0 mA/ml (4), respectively. The initial fast rising phase of the Ca2+ transients was not modified. (-)-Bay K 8644 slowed the time constant of decay of the transients by 57 +/- 6 ms. In other experimental conditions, Ca2+ release from the sarcoplasmic reticulum (SR) was impaired with repetitive stimulation in 1 mM [EGTA]i-containing fibers. Under those circumstances, Ca2+ transients directly followed the time integra...Continue Reading

References

Apr 28, 1978·Annals of the New York Academy of Sciences·C C Ashley
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Citations

May 8, 1995·Biochimica Et Biophysica Acta·W MelzerH C Lüttgau
Nov 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zoita AndronacheWerner Melzer
Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zoita AndronacheWerner Melzer
Jan 1, 1994·The Journal of General Physiology·J García, K G Beam
Oct 5, 2001·The Journal of General Physiology·P SzentesiL Csernoch
Mar 19, 2003·The Journal of General Physiology·W K ChandlerS M Baylor
Dec 17, 2003·The Journal of General Physiology·R P Schuhmeier, W Melzer
Aug 29, 2007·The Journal of General Physiology·Stephen M Baylor, Stephen Hollingworth
Jan 1, 1987·The Journal of General Physiology·M IrvingW K Chandler
Jan 1, 1987·The Journal of General Physiology·J MaylieW K Chandler
May 1, 1991·The Journal of General Physiology·J GarcíaE Stefani
May 1, 1991·The Journal of General Physiology·G SzücsL Kovács
May 9, 1998·Journal of Applied Physiology·S P CairnsD S Loiselle

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