PMID: 11311317Apr 20, 2001Paper

Relationship between NADP-specific isocitrate dehydrogenase and glutathione peroxidase in aging rat skeletal muscle

Mechanisms of Ageing and Development
John M Lawler, S R Demaree

Abstract

The glutathione peroxidase (GPX) system detoxifies hydroperoxides in cells and uses NADPH to regenerate reduced glutathione. Enzymatic sources of NADPH in skeletal muscle include NADP-specific isocitrate dehydrogenase (ICDP), glucose-6-phosphate dehydrogenase (G6PD), and malic enzyme (ME). Our purpose was to explore the relationship in skeletal muscle between GPX and ICDP along with other NADPH-generating enzymes as a function of progressive age and muscle fiber-type. Soleus (SOL), red gastrocnemius (RG), and white gastrocnemius (WG) muscles were extracted from Fischer-344 rats of three different ages: 4 months old (Y); 18 months old (M); and 24 months old (O). Assays were conducted to determine activities of GPX, ICDP, G6PD, and ME along with levels of lipid hydroperoxides. GPX activities were significantly greater in RG and WG of old rats than in younger. ICDP activities were higher in the WG of old and middle aged rats when compared to young adults. GPX and ICDP activities exhibited similar differences among the muscles tested (SOL>RG>WG). In contrast, G6PD and ME activities were not significantly different across muscles. G6PD activities increased in RG with age, but were well over an order of magnitude lower than ICDP in a...Continue Reading

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Citations

Feb 7, 2002·Experimental Gerontology·Eli CarmeliAbraham Z Reznick
Jun 27, 2003·Free Radical Biology & Medicine·John M LawlerScott R Demaree
Nov 23, 2006·Antioxidants & Redox Signaling·Orly WeinrebMoussa B H Youdim
Oct 17, 2008·Physiological Reviews·Scott K Powers, Malcolm J Jackson
Jan 10, 2002·Biochemical and Biophysical Research Communications·John M LawlerScott Demaree
Apr 10, 2016·The Journal of Physiology·John M LawlerJeffrey M Hord
Jun 7, 2019·The Journal of Experimental Biology·Kaitlin N AllenAllyson G Hindle
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Ricarda CarlisleLynn Harrison
Nov 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aiwen JiangHonglin Liu
May 2, 2003·American Journal of Physiology. Cell Physiology·B RamamurthyL Larsson

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