Relationship between OPA1 and cardiolipin in mitochondrial inner-membrane fusion

Biochimica Et Biophysica Acta. Bioenergetics
Tadato BanNaotada Ishihara

Abstract

Mitochondria are highly dynamic organelles that undergo frequent fusion and fission. The large GTPase optic atrophy 1 (OPA1) is identified as a core component of inner membrane (IM) fusion. OPA1 exists as the membrane-anchored L-OPA1 and the proteolytically cleavage soluble S-OPA1. Recently, we showed that OPA1 and mitochondria-localized lipid cardiolipin (CL) cooperate in heterotypic IM fusion [Ban et al., Nat. Cell Biol. 19 (2017) 856-863]. We reconstituted an in vitro membrane fusion reaction using purified human L-OPA1 and S-OPA1 expressed in silkworm and found that L-OPA1 on one side of the membrane and CL on the other side were sufficient for mitochondrial fusion. L-OPA1 is the major fusion-prone factor in heterotypic fusion. However, the role of S-OPA1 remains unknown as S-OPA1 promoted L-OPA1-dependent heterotypic membrane fusion and homotypic CL-containing membrane fusion, but S-OPA1 alone was not sufficient for heterotypic membrane fusion. L-OPA1- and CL-mediated heterotypic mitochondrial fusion was confirmed in living cells, but tafazzin (Taz1), the causal gene product of Barth syndrome, was not essential for mitochondrial fusion. Taz1-dependent CL maturation might have other roles in the remodeling of mitochondrial ...Continue Reading

Citations

Oct 6, 2019·Annual Review of Pathology·David C Chan
Jun 25, 2020·Journal of Cell Science·Richard G LeeAleksandra Filipovska
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Yeojin Moon, Youngsoo Jun
Dec 14, 2018·Antioxidants·Hakjoo Lee, Yisang Yoon
Nov 26, 2020·Molecular Biology of the Cell·Ruohan WangDavid C Chan
Feb 13, 2021·Cellular and Molecular Life Sciences : CMLS·Mitali ShahVaishnavi Ananthanarayanan
Jun 15, 2019·Journal of Proteome Research·Cinzia BoccaArnaud Chevrollier

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