PMID: 8605090Mar 1, 1996Paper

Relationship between p53 status and radiosensitivity in human tumour cell lines

British Journal of Cancer
E SilesJ M Ruiz de Almodóvar

Abstract

We examined the relationship between p53 levels before and after irradiation, radiation-induced cell cycle delays, apoptotic cell death and radiosensitivity in a panel of eight human tumour cell lines. The cell lines differed widely in their clonogenic survival after radiation, (surviving fraction at 2 Gy: SF2=0.18-0.82). Constitutive p53 protein levels varied from 2.2 +/- 0.4 to 6.3 +/- 0.3 optical density units (OD) per 10(6) cells. p53 after irradiation (6 Gy) also varied between the cell lines, ranging from no induction to a 1.6-fold increase in p53 levels 4 h after treatment. p53 function was also assessed by G1 cell cycle arrest after irradiation. The cellular response to radiation, measured as G0/G1 arrest, and the induction of apoptosis were in good agreement. However, a trace amount of DNA ladder formation was found in two cell lines lacking G1 arrest. Overall cellular radiosensitivity correlated well with the level of radiation-induced G1 arrest (correlation coefficient r=0.856; P=0.0067), with p53 constitutive levels (r=0.874, P=0.0046), and with p53 protein fold induction (r=-0.882, P=0.0038). Our data suggest that (1) the constitutive p53 level, (2) G1 arrest after irradiation, or (3) the p53 protein response to ra...Continue Reading

Citations

Jul 17, 1997·International Journal of Cancer. Journal International Du Cancer·M T ValenzuelaJ M Ruiz de Almodóvar
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Aug 17, 2018·Molecular Cancer·Virgínea de Araujo FariasJosé Mariano Ruiz de Almodóvar
Nov 16, 2004·The Journal of Urology·Ke-Hung TsuiHorng-Heng Juang
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