Relationship between paxillin and myosin phosphorylation during muscarinic stimulation of smooth muscle

The American Journal of Physiology
D MehtaSusan J Gunst

Abstract

The tyrosine phosphorylation of paxillin increases in association with force development during tracheal smooth muscle contraction, suggesting that paxillin plays a role in the contractile activation of smooth muscle [Z. L. Wang, F. M. Pavalko, and S. J. Gunst. Am. J. Physiol. 271 (Cell Physiol. 40): C1594-C1602, 1996]. We compared the Ca2+ sensitivity of the tyrosine phosphorylation of paxillin and myosin light chain (MLC) phosphorylation in tracheal muscle and evaluated whether MLC phosphorylation is necessary to induce paxillin phosphorylation. Ca(2+)-depleted muscle strips were stimulated with 10(-7)-10(-4) M acetylcholine (ACh) in 0,0.05, 0.1, or 0.5 mM extracellular Ca2+. In the absence of extracellular Ca2+, 10(-4) M ACh induced a maximal increase in paxillin phosphorylation without increasing MLC phosphorylation or force. Increases in extracellular Ca2+ concentration did not further increase paxillin phosphorylation. However, during stimulation with 10(-6) M ACh, paxillin phosphorylation increased with increases in extracellular Ca2+ concentration. We conclude that the tyrosine phosphorylation of paxillin can be stimulated by signaling pathways that do not depend on Ca2+ mobilization and that the activation of contracti...Continue Reading

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Citations

Mar 14, 2001·American Journal of Physiology. Cell Physiology·D D Tang, S J Gunst
Jul 18, 2002·The Journal of Physiology·Dale D TangSusan J Gunst
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Luke J Janssen
Mar 19, 2008·Journal of Muscle Research and Cell Motility·Ozgur OgutFrank V Brozovich
Jul 27, 2000·American Journal of Physiology. Cell Physiology·D MehtaS J Gunst
Jun 13, 2001·American Journal of Physiology. Cell Physiology·D SulR F Coburn
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H YoshimuraD O Warner
Jun 29, 2007·American Journal of Physiology. Cell Physiology·Christopher M RemboldShaojie Han
Oct 16, 2009·American Journal of Physiology. Cell Physiology·Ankit D Tejani, Christopher M Rembold
Apr 8, 2009·The Journal of Biological Chemistry·Hai-Lei DingKristine E Kamm
May 11, 2006·Pflügers Archiv : European journal of physiology·Albert Y RheeFrank V Brozovich
Sep 28, 2013·Journal of Applied Physiology·Bo LanChun Y Seow
Jun 10, 2017·Journal of Cell Science·Pasquale ChitanoChun Y Seow
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·T KaiD O Warner
Dec 19, 2017·Journal of Immunology Research·René HuberKorbinian Brand
Feb 1, 2003·Archives of Biochemistry and Biophysics·Albert Y Rhee, Frank V Brozovich
Jun 5, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ibrahima DiagneSheila G Haworth

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